SPONTANEOUS, HERITABLE COLITIS IN A NEW SUBSTRAIN OF C3H/HEJ MICE
SPONTANEOUS, HERITABLE COLITIS IN A NEW SUBSTRAIN OF C3H/HEJ MICE
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DOI：
10.1016/0016-5085(94)90813-3
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发表时间：
1994-12-01
期刊：
影响因子：
29.4
通讯作者：
BIRKENMEIER, EH
中科院分区：
文献类型：
--
作者：
SUNDBERG, JP;ELSON, CO;BIRKENMEIER, EH
背景/目的：杰克逊实验室的C3H/HeJ小鼠由于出现软粪便、肛周溃疡和右侧结肠炎而定期被扑杀。目前还没有分离出任何病原体。目前这项研究的目标是建立一种这种疾病的高发病率亚株。方法：选用C3H/HeJ小鼠，雌雄各半。对所产生的家系中的216只小鼠的临床、病理、微生物学和遗传学特征进行了表征。结果：一名严重患病的女性与一名正常男性杂交，产生了一种新的亚株，命名为C3H/HeJBir，右侧结肠炎的发生率很高。组织学上，病变主要发生在盲肠和近端结肠，以急性和慢性炎症、隐窝脓肿、溃疡、再生性增生和粘膜下疤痕为特征。这种结肠炎在3-6周时达到高峰；然而，在1岁以上的动物中也零星发现了类似的疾病。肛门直肠交界处的小病变在一生中很常见。对病原体的广泛搜索没有结果。对C3H/HeJBir小鼠的遗传分析表明，该病是作为数量性状遗传的。结论：C3H/HeJBir小鼠发生了一种自发的、可遗传的特发性炎症性肠病，为该疾病的遗传学和免疫学研究提供了有价值的资源。
Background/Aims: C3H/HeJ mice at the Jackson Laboratory have periodically been culled because of the occurrence of soft feces, perianal ulceration, and right-sided colitis. No pathogens have been isolated. The goal of the current study was to establish a substrain with a high incidence of this disease. Methods: Affected male and female C3H/HeJ mice were bred. The clinical, pathological, microbiological, and genetic features of 216 mice of the resulting pedigree were characterized. Results: A severely affected female crossed with a normal male resulted in a new substrain, denoted C3H/HeJBir, with a high incidence of right-sided colitis. Histologically, lesions occurred primarily in the cecum and proximal colon, characterized by acute and chronic inflammation, crypt abscesses, ulcerations, regenerative hyperplasia, and submucosal scarring. Such colitis peaked at 3-6 weeks; however, similar disease was found sporadically in animals more than 1 year of age. Small lesions at the anorectal junction were common throughout life. An extensive search for pathogens was negative. Genetic analysis of C3H/HeJBir mice suggested that the disease was inherited as a quantitative trait. Conclusions: C3H/HeJBir mice develop a spontaneous, heritable form of idiopathic inflammatory bowel disease and will be a valuable resource for genetic and immunologic studies of this disease.