PLASMA-CONCENTRATIONS OF DELTA-9-TETRAHYDROCANNABINOL IN DAMS AND FETUSES FOLLOWING ACUTE OR MULTIPLE PRENATAL DOSING IN RATS
PLASMA-CONCENTRATIONS OF DELTA-9-TETRAHYDROCANNABINOL IN DAMS AND FETUSES FOLLOWING ACUTE OR MULTIPLE PRENATAL DOSING IN RATS
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DOI：
10.1016/0024-3205(89)90380-9
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发表时间：
1989-01-01
期刊：
影响因子：
6.1
通讯作者：
FICO, T
中科院分区：
文献类型：
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作者：
HUTCHINGS, DE;MARTIN, BR;FICO, T
用Delta9-四氢大麻酚(THC)灌胃给孕鼠，研究剂量水平和给药方案对母鼠和胎鼠血药浓度的影响。在妊娠的最后两周，两个多剂量组分别给予15 mg/kg或50 mg/kg的Delta-9-THC，每天一次。两个急性组的剂量与上述相同，但仅在妊娠最后一天给药一次。最后一次给药60min后，断头处死所有母鼠及其胎儿，采集血液，离心法分离血浆。用气相色谱-质谱法对血浆中的β-9-THC进行了定量测定。各组大鼠的血药浓度随剂量的变化而变化，且多次给药的血药浓度高于急性给药，尤其是在高剂量时。在这些胎儿中，血浆浓度大约是大坝中发现的浓度的10%。同样，来自高剂量、多剂量水坝的胎儿产生的浓度也明显更高。这些发现被讨论与其他研究胎盘转移的增量-9-THC和影响出生后发育。
Delta-9-tetrahydrocannabinol (THC) was administered by gastric intubation to pregnant rats to study the effects of dose-level and dosing regimen on plasma concentration in dams and fetuses. Two multiple-dose groups were administered either 15 or 50 mg/kg of delta-9-THC once daily during the last two weeks of gestation. Two acute groups were administered the same dose as above but only once on the last day of gestation. Sixty min after receiving the last dose all dams and their fetuses were sacrificed by decapitation, blood collected, centrifuged and plasma removed. Quantitative measurement of delta-9-THC in plasma was carried out using GS/MS. Among the dams, plasma concentrations covaried with dose and multiple dosing produced higher concentrations than acute, especially at the high dose. Among the fetuses, plasma concentrations were approximately 10% of those found in the dams. The fetuses from the high, multiple-dose dams similarly yielded significantly higher concentrations. These findings are discussed with respect to other studies of the placental transfer of delta-9-THC and effects on postnatal development.