Site-specific implantation in the milky spots of malignant cells in peritoneal dissemination: immunohistochemical observation in mice inoculated intraperitoneally with bromodeoxyuridine-labelled cells.
Site-specific implantation in the milky spots of malignant cells in peritoneal dissemination: immunohistochemical observation in mice inoculated intraperitoneally with bromodeoxyuridine-labelled cells.
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DOI：
10.1038/bjc.1995.95
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发表时间：
1995-03
影响因子：
8.8
通讯作者：
Yamasaki, J.
中科院分区：
文献类型：
--
作者：
Tsujimoto, H.;Takahashi, T.;Hagiwara, A.;Shimotsuma, M.;Sakakura, C.;Osaki, K.;Sasaki, S.;Shirasu, M.;Sakakibara, T.;Ohyama, T.;Sakuyama, A.;Ohgaki, M.;Imanishi, T.;Yamasaki, J.
为了研究癌细胞在腹膜扩散中的定位种植，我们用溴脱氧尿嘧啶核苷(BrdU)标记的小鼠P388白血病细胞接种CDF1小鼠，然后用抗BrdU抗体用免疫组织化学方法观察细胞在大网膜内的分布。我们发现BrdU标记的细胞选择性地渗入到大网膜的乳斑中。此外，我们将每只小鼠10(5)、10(6)和10(7)个细胞的BrdU标记的P388细胞接种到CDF1小鼠三组，每组10只，然后通过计数每单位面积的BrdU标记细胞在大网膜中的每个乳斑和非乳斑部位的数量来量化BrdU标记细胞的分布。每只小鼠接种10(5)、10(6)和10(7)BrdU标记的P388细胞，在乳斑部位分别产生15.8+/-13.3、120+/-46.5和504+/-208个细胞mm-2和9.14×10(-3)+/-1.58×10(-2)个。非银斑点分别为1.14×10(-1)+/-7.82×10(-2)和7.07×10(-1)+/-5.98×10(-1)cell mm-2。银斑区与非银斑区的平均标记细胞数之比分别为1728：1、1049：1和713：1。在所有病例中，在乳斑和非乳斑之间，BrdU标记的细胞数mm-2有统计学意义上的差异。然而，随着接种细胞数量的增加，这一比例下降。此外，我们还将B-16PC黑色素瘤细胞接种于C57/BL小鼠体内，从宏观和微观上观察了细胞的分布情况。B-16PC黑色素瘤细胞也被发现优先渗透到大网膜的乳白色斑点。这些结果表明，在腹膜扩散的早期阶段，接种于腹膜内的癌细胞特异性地渗入乳斑。
To investigate the site-specific implantation of cancer cells in peritoneal dissemination, we inoculated CDF1 mice intraperitoneally with mouse P388 leukaemia cells labelled with bromodeoxyuridine (BrdU) and then observed immunohistochemically the distribution of the cells in the greater omentum taken from the mice using an anti-BrdU antibody. We found the BrdU-labelled cells infiltrating selectively into the milky spots in the omentum. Furthermore, we intraperitoneally inoculated the BrdU-labelled P388 cells at 10(5), 10(6) and 10(7) cells per mouse into three groups of ten CDF1 mice and then quantified the distribution of the BrdU-labelled cells by counting the number of the labelled cells per unit area at each milky spot and non-milky spot site in the omentum. Inoculations of 10(5), 10(6) and 10(7) BrdU-labelled P388 cells per mouse resulted in 15.8 +/- 13.3, 120 +/- 46.5 and 504 +/- 208 cells mm-2 respectively in the milky spot sites and 9.14 x 10(-3) +/- 1.58 x 10(-2), 1.14 x 10(-1) +/- 7.82 x 10(-2) and 7.07 x 10(-1) +/- 5.98 x 10(-1) cells mm-2 respectively in the non-milky spot sites. The ratios of the mean labelled cell numbers in the milky spot sites vs those in the non-milky spot sites were 1728:1, 1049:1 and 713:1 respectively. In all cases, there were statistically significant differences in the number of BrdU-labelled cells mm-2 between milky spot sites and non-milky spot sites. However, the ratios decreased as the numbers of inoculated cells increased. In addition, we inoculated C57/BL mice intraperitoneally with B-16 PC melanoma cells, which were easily differentiated from the other cells by the intrinsic black melanin, and examined the distribution of the cells macro- and microscopically. The B-16 PC melanoma cells were also found to be infiltrating preferentially into the milky spots in the omentum. These results suggest that cancer cells seeded intraperitoneally specifically infiltrate the milky spots in the early stages of peritoneal dissemination.
影响因子：
3.6
作者：
SHIMOTSUMA, M;TAKAHASHI, T;DUX, K
通讯作者：
DUX, K
影响因子：
56.9
作者：
GRATZNER, HG
通讯作者：
GRATZNER, HG
影响因子：
1.6
作者：
DUX, K;SHIMOTSUMA, M;SIMPSONMORGAN, MW
通讯作者：
SIMPSONMORGAN, MW