Chromosomal location and structure of the operon encoding peptide-chain-release factor 2 of Escherichia coli.
Chromosomal location and structure of the operon encoding peptide-chain-release factor 2 of Escherichia coli.
复制标题
编码大肠杆菌肽链释放因子 2 的操纵子的染色体位置和结构。
DOI：
10.1073/pnas.85.15.5620
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发表时间：
1988
影响因子：
11.1
通讯作者：
Y. Nakamura
中科院分区：
文献类型：
--
作者：
K. Kawakami;Y. Jönsson;G. Björk;H. Ikeda;Y. Nakamura
prfB基因编码大肠杆菌肽链释放因子2，在UGA和UAA密码子处催化翻译终止。通过测序鉴定，该基因位于大肠杆菌染色体的62分钟区域。prfB基因后面是一个编码57,603-Da蛋白的开放阅读框。这个下游开放阅读框被鉴定为herC，这是一个由抑制突变定义的基因，它可以恢复ColE1质粒突变体的复制。RNA印迹杂交和体内转录本S1核酸酶保护分析表明，prfB和herC在大肠杆菌遗传图谱上沿逆时针方向共转录成2800个碱基的转录本。因此，我们将这两个基因称为prfB-herC操纵子。有数据表明，鼠伤寒沙门菌中抑制UGA终止的突变supK是沙门菌释放因子2的结构基因。本文讨论了prfB-herC操纵子的翻译控制以及这些基因与tRNA甲基转移酶的关系。
The prfB gene encodes peptide-chain-release factor 2 of Escherichia coli, which catalyzes translation termination at UGA and UAA codons. The gene, identified by sequencing, is located at the 62-min region of the E. coli chromosome. The prfB gene is followed by an open reading frame encoding a 57,603-Da protein. This downstream open reading frame was identified as herC, a gene defined by a suppressor mutation that restores replication of a ColE1 plasmid mutant. RNA blot hybridization and S1 nuclease protection analyses of in vivo transcripts showed that prfB and herC are cotranscribed into a 2800-base transcript in the counterclockwise direction with respect to the E. coli genetic map. Thus, we refer to the two genes as the prfB-herC operon. Data are presented that suggest that supK, a mutation in Salmonella typhimurium that suppresses UGA termination, is the structural gene for Salmonella release factor 2. Translation control within the prfB-herC operon and the relationship of these genes to a tRNA methyltransferase are discussed.
影响因子：
4.3
作者：
H. Sambrook
通讯作者：
H. Sambrook