Pseudomonas azotifigens sp nov., a novel nitrogen-fixing bacterium isolated from a compost pile
Pseudomonas azotifigens sp nov., a novel nitrogen-fixing bacterium isolated from a compost pile
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DOI：
10.1099/ijs.0.63586-0
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发表时间：
2005-07-01
影响因子：
2.8
通讯作者：
Nakamura, A
中科院分区：
文献类型：
--
作者：
Hatayama, K;Kawai, S;Nakamura, A
从日本的堆肥堆中分离到一株固氮菌，命名为6 H33 b（T）。根据该菌株在低氧浓度（2-4%）下的乙炔还原活性，检测了该菌株的固氮酶活性。对该菌株中负责固氮的基因nifH和nifD的分析表明，与施氏假单胞菌A15（A1501）的基因关系密切。基于16 S rRNA基因序列的序列相似性搜索表明，菌株6 H33 b（T）属于狭义假单胞菌属，与印度假单胞菌的相似性最高（97.3%）。通过对6 H33 b（T）与印度假单胞菌（P. indica）和狭义假单胞菌（Pseudomonasensustricto）的一些分类学特征的比较，表明6 H33 b（T）与印度假单胞菌（P. indica）的区别在于具有固氮能力，不具有硝酸盐还原和反硝化能力，以及对某些糖和有机酸的利用。系统发育分析和DNA-DNA杂交结果也表明，菌株6 H33 b（T）代表了一个不同于P. indica的种。根据这些结果，提出菌株6 H33 b（T）（=ATCC BAA-1049（T）=JCM 12708（T））被分类为狭义假单胞菌属的新种的模式菌株，命名为固氮假单胞菌新种。
A nitrogen-fixing bacterium, designated strain 6H33b(T), was isolated from a compost pile in Japan. The nitrogenase activity of this strain was detected based on its acetylene-reducing activity under low oxygen concentrations (2-4%). An analysis of the genes responsible for nitrogen fixation in this strain, nifH and nifD, indicated a close relationship to those of Pseudomonas stutzeri A15 (A1501). Sequence similarity searches based on the 16S rRNA gene sequences showed that strain 6H33b(T) belongs within the genus Pseudomonas sensu stricto; closest similarity was with Pseudomonas indica (97.3%). A comparison of several taxonomic characteristics of 6H33b(T) with those of P. indica and some type strains of the genus Pseudomonas sensu stricto indicated that 6H33b(T) could be distinguished from P. indica based on the presence of nitrogen fixation ability, the absence of nitrate reduction and denitrification abilities and the utilization of some sugars and organic acids. Phylogenetic analyses and the results of DNA-DNA hybridization experiments also indicated that strain 6H33b(T) represents a species distinct from P. indica. From these results, it is proposed that strain 6H33b(T) (=ATCC BAA-1049(T)=JCM 12708(T)) is classified as the type strain of a novel species of the genus Pseudomonas sensu stricto under the name Pseudomonas azotifigens sp. nov.