Concordance between direct and indirect measurements of free 25-OH vitamin D.
Concordance between direct and indirect measurements of free 25-OH vitamin D.
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DOI：
10.1016/j.cca.2017.10.016
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发表时间：
2017-12
期刊：
影响因子：
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通讯作者：
P. Peris;X. Filella;A. Monegal;N. Guañabens;L. Foj;M. Bonet;D. Boquet;E. Casado;D. Cerdá
中科院分区：
文献类型：
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作者：
P. Peris;X. Filella;A. Monegal;N. Guañabens;L. Foj;M. Bonet;D. Boquet;E. Casado;D. Cerdá
目前，比较游离维生素D的定量测定方法（直接测定法[direct 25-OHDF]和计算估计法[calculate 25-OHDF]）的数据很少。本研究的目的是分析这两种25- ohdf分析方法（直接与计算）之间的一致性。方法对173例35 ~ 45岁健康女性血清总25-OHDT、维生素D结合蛋白（DBP） （R&D Systems ELISA）、计算25-OHDF（DBP、白蛋白和25-OHDT）和直接25-OHDF（DIAsource ELISA）进行测定。采用Bland-Altman图和总偏差指数（TDI）评价一致性。结果25-OHDFin计算值和直接值的平均值分别为5.27±2.5 pg/mL和3.83±1.01 pg/mL。我们发现，与使用的方法无关，与维生素D缺乏症患者和非维生素D缺乏症患者相比，25-OHDFon的值显著降低。由Bland-Altman图评估的总偏差指数显示两个测量值的一致性较低。只有低25- ohdf水平是一致的。结论25- ohdf两种分析方法的一致性较低，存在浓度依赖偏倚。需要进一步的研究来明确25- ohdf测量的参考值和适应症。
At present, data comparing the quantification methods for measurement of free vitamin D (direct assay [direct 25-OHDF] and estimated by calculation [calculated 25-OHDF]), are scarce. The aim of this study was to analyse the concordance between these two methods of 25-OHDFanalysis (direct vs. calculated).MethodsSerum values of total 25-OHD (25-OHDT), vitamin D binding protein (DBP) (by R&D Systems ELISA), calculated 25-OHDF(by DBP, albumin and 25-OHDT) and direct 25-OHDF(by DIAsource ELISA) were analysed in 173 healthy women (aged 35–45 years). Assessment of concordance was evaluated by the Bland-Altman plot and the total deviation index (TDI).ResultsThe mean values of calculated and direct 25-OHDFin these subjects were 5.27 ± 2.5 and 3.83 ± 1.01 pg/mL, respectively. We found significantly lower values of 25-OHDFon comparing subjects with and without vitamin D deficiency, independently of the method used. The total deviation index evaluated by the Bland-Altman plot showed low concordance for both measurements. Only low 25-OHDFlevels were concordant.ConclusionsThis study shows that the concordance between these two methods of 25-OHDFanalysis is low and has a concentration dependent bias. Further studies are necessary to clarify the reference values and the indications for 25-OHDFmeasurement.