Racial Differences in Paraoxonase-1 (PON1): A Factor in the Health of Southerners?
Racial Differences in Paraoxonase-1 (PON1): A Factor in the Health of Southerners?
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DOI：
10.1289/ehp.0900569
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发表时间：
2009-08-01
影响因子：
10.4
通讯作者：
Chambers, Janice E.
中科院分区：
文献类型：
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作者：
Davis, Kimberly A.;Crow, J. Allen;Chambers, Janice E.
背景：美国南部（不包括佛罗里达州）的心血管疾病（CVD）年龄调整率全国最高，非裔美国人的 CVD 患病率高于白种人。对氧磷酶-1 (PON1) 是一种与高密度脂蛋白颗粒相关的酶，参与氧化脂质的水解（从而防止动脉粥样硬化）和有机磷酸酯的水解。较高的对氧磷酶活性与较低的动脉粥样硬化风险相关。 目的：在这项研究中，我们通过商业获得的血库血清，对来自美国南部的 200 名两个种族的成年男性和女性（每个种族/性别类别各 50 名）的功能性 PON1(Q192R) 多态性（通过重氮磷酶与对氧磷酶比率评估 PON 状态进行评估）的分布和 PON1 活性水平进行了特征分析方法：我们使用血清分光光度法测定 PON1 状态、芳基酯酶活性（乙酸苯酯水解）以及可替宁和 C 反应蛋白 (CRP) 水平。结果：与白种人相比，非裔美国人对氧磷酶活性较高，但重氮磷酶活性较低，这与非裔美国人的功能性基因型 QQ 比例较低（QQ 15%、QR 34%、RR 44%、 7% 不确定），高于白人（QQ 60%，QR 31%，RR 7%，2% 不确定）。可替宁水平表明，所有样本均来自非吸烟者，非裔美国人的 CRP 水平高于白人，女性高于男性。 CRP 水平与对氧磷酶活性没有关联。结论：这些数据提供了初步观察结果，可用于描述美国南部人群（更具体地说是南部非洲裔美国人）较差的心血管健康状况。
BACKGROUND: The southern United States (excluding Florida) has the highest age-adjusted rate of cardiovascular disease (CVD) in the country, with African Americans having a higher prevalence of CVD than Caucasians. Paraoxonase-1 (PON1), an enzyme associated with high-density lipoprotein particles, participates both in the hydrolysis of oxidized lipids (thus protecting against atherosclerosis) and in the hydrolysis of organophosphates. Higher paraoxonase activity has been associated with lower risk of atherosclerosis.OBJECTIVES: In this study we characterized the distribution of the functional PON1(Q192R) Polymorphisms (PON status as assessed by diazoxonase to paraoxonase ratios) and the PON1 activity levels in 200 adult males and females of both races (50 in each race/sex class) from the southern United States from commercially obtained blood bank serum samples.METHODS: We used spectrophotometric methods with serum to determine PON1 status, arylesterase activities (phenyl acetate hydrolysis), and levels of cotinine and C-reactive protein (CRP).RESULTS: African Americans had higher paraoxonase activities but lower diazoxonase activities than did Caucasians, consistent with African Americans having a lower proportion of the functional genotype QQ (QQ 15%, QR 34%, RR 44%, 7% indeterminate), than did Caucasians (QQ 60%, QR 31%, RR 7%, 2% indeterminate). Cotinine levels indicated that all samples came from nonsmokers and that CRP levels were higher in African Americans than in Caucasians and higher in females than in males. CRP levels showed no association with paraoxonase activities.CONCLUSIONS: These data present initial observations for use in characterizing the poorer cardiovascular health status of the population in the southern United States and more specifically southern African Americans.