Comparison of in vitro hepatogenic differentiation potential between various placenta-derived stem cells and other adult stem cells as an alternative source of functional hepatocytes
Comparison of in vitro hepatogenic differentiation potential between various placenta-derived stem cells and other adult stem cells as an alternative source of functional hepatocytes
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DOI：
10.1016/j.diff.2012.05.007
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发表时间：
2012-10-01
期刊：
影响因子：
2.9
通讯作者：
Kim, Gi Jin
中科院分区：
文献类型：
--
作者：
Lee, Hyun-Jung;Jung, Jieun;Kim, Gi Jin
间充质干细胞（MSCs）是再生医学中细胞治疗的强大来源。获得有效的干细胞衍生肝细胞的能力将改善肝病的细胞治疗。近年来，各种胎盘来源的干细胞（PDSC）取决于胎盘的定位已被建议作为干细胞的替代来源，类似于骨髓来源的MSC（BM-MSC）和脂肪来源的MSC（AD-MSC）。然而，对不同来源的骨髓间充质干细胞向肝细胞分化潜能的比较研究还很缺乏。因此，我们研究比较PDSCs与BM-MSCs、AD-MSCs和UCB-MSCs向肝细胞分化的潜力。从人足月胎盘、脂肪组织和脐带血中分离出多株间充质干细胞，经中胚层分化后，采用定量逆转录-PCR（RT-PCR）和特殊染色法对分离的间充质干细胞和BM-MSCs进行鉴定。采用RT-PCR、PAS染色、ICG摄取试验、白蛋白表达、尿素生成和细胞因子测定等方法比较PDSCs与AD-MSCs、UCB-MSCs和BM-MSCs的肝细胞生成潜能。从不同组织中分离的MSC均具有相似的MSC特性。然而，PDSCs的增殖能力和分化的PDSCs中肝源性标志物的表达高于其他MSC。有趣的是，肝细胞生长因子（HGF）的表达增加后，肝细胞分化的PDSCs。有趣的是，干细胞因子（SCF）的表达在绒毛膜板来源的骨髓间充质干细胞，PDSC之一，显着高于其他PDSC。两者合计，本研究的结果表明，从各种成人组织中分离的MSC可以诱导进行体外肝分化，PDSCs可能具有最大的肝分化和增殖的潜力。因此，PDSC可用作肝脏疾病细胞治疗的干细胞来源。版权所有（c）2012国际分化学会。Elsevier B. V.出版，保留所有权利。
Mesenchymal stem cells (MSCs) are powerful sources for cell therapy in regenerative medicine. The capability to obtain effective stem cell-derived hepatocytes would improve cell therapy for liver diseases. Recently, various placenta-derived stem cells (PDSCs) depending on the localization of placenta have been suggested as alternative sources of stem cells are similar to bone marrow-derived MSC (BM-MSCs) and adipose-derived MSC (AD-MSCs). However, comparative studies for the potentials of the hepatogenic differentiation among various MSCs largely lacking. Therefore, we investigated to compare the potentials for hepatogenic differentiation of PDSCs with BM-MSCs, AD-MSCs, and UCB-MSCs. Several MSCs were isolated from human term placenta, adipose tissue, and umbilical cord blood and characterized isolated MSCs and BM-MSCs was performed by quantitative reverse transcription-PCR (RT-PCR) and special stains after mesodermal differentiation. The hepatogenic potential of PDSCs was compared with AD-MSCs, UCB-MSCs, and BM-MSCs using RT-PCR, PAS stain, ICG up-take assays, albumin expression, urea production, and cytokine assays. MSCs isolated from different tissues all presented similar characteristics of MSCs. However, the proliferative potential of PDSCs and the expression of hepatogenic markers in differentiated PDSCs were higher than other MSCs. Interestingly, the expression of hepatocyte growth factor (HGF) increased in PDSCs after hepatogenic differentiation. Interestingly, stem cell factor (SCF) expression in chorionic plate-derived MSCs, one of the PDSCs, was significantly higher than in the other PDSCs. Taken together, the results of the present study suggest that MSCs isolated from various adult tissues can be induced to undergo hepatogenic differentiation in vitro, and that PDSCs may have the greatest potential for hepatogenic differentiation and proliferation. Therefore, PDSCs could be used as a stem cell source for cell therapy in liver diseases. Copyright (c) 2012 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.