Biosynthetic pathway of insect juvenile hormone III in cell suspension cultures of the sedge Cyperus iria
Biosynthetic pathway of insect juvenile hormone III in cell suspension cultures of the sedge Cyperus iria
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DOI：
10.1104/pp.010264
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发表时间：
2001-10-01
期刊：
影响因子：
7.4
通讯作者：
Tobe, SS
中科院分区：
文献类型：
--
作者：
Bede, JC;Teal, PEA;Tobe, SS
在大多数昆虫物种中，保幼激素调节关键的生理过程，如变态和繁殖。在昆虫中，这些倍半萜类化合物是由大脑后内分泌器官--腮体通过经典的甲氧戊酸(MVA)途径合成的。其中一种化合物，保幼激素III(JH111)，也在莎草中被鉴定出来。在高等植物中，倍半萜骨架的生物合成可通过两条不同的途径进行：MVA途径或2C-甲基赤藓糖醇4-磷酸途径或两者的组合。通过悬浮培养的方法研究了JH III在植物体内的生物合成途径。酶抑制和标记研究结果表明，JH III的倍半萜骨架的生物合成是通过MVA途径进行的。抑制剂和前体饲喂研究也表明，虹彩弯孢杆菌JH III生物合成的后期步骤类似于昆虫的途径，JH III生物合成的最终酶反应是由细胞色素P(450)单加氧酶催化的。
In most insect species, juvenile hormones regulate critical physiological processes such as metamorphosis and reproduction. In insects, these sesquiterpenoids are synthesized by retrocerebral endocrine organs, the corpora allata, via the classical mevalonate (MVA) pathway. One of these compounds, juvenile hormone III (JH 111), has also been identified in the sedge Cyperus iria. In higher plants, biosynthesis of the sesquiterpenoid backbone may proceed through two distinct pathways: the MVA pathway or the 2C-methyl erythritol 4-phosphate pathway or through a combination of both pathways. Cell suspension cultures of C. iria were used to elucidate the biosynthetic pathway of JH III in the plant. Enzyme inhibition and labeling studies conclusively demonstrated that the biosynthesis Of the sesquiterpenoid backbone of JH III proceeds via the MVA pathway. Inhibitor and precursor feeding studies also suggest that later steps of JH III biosynthesis in C. iria are similar to the insect pathway and that the final enzymatic reaction in JH III biosynthesis is catalyzed by a cytochrome P(450) monooxygenase.