Cell-in-cell Structures Formed between Human Cancer Cell Lines and the Cytotoxic Regulatory T-cell Line HOZOT
Cell-in-cell Structures Formed between Human Cancer Cell Lines and the Cytotoxic Regulatory T-cell Line HOZOT
复制标题
人癌细胞系与细胞毒性调节性T细胞系HOZOT之间形成的细胞中细胞结构
DOI：
10.1093/jmcb/mjq002
复制
发表时间：
2010-06-01
影响因子：
5.5
通讯作者：
Kibata, Masayoshi
中科院分区：
文献类型：
--
作者：
Takeuchi, Makoto;Inoue, Toshiya;Kibata, Masayoshi
我们之前建立了一种新的细胞系，称为HOZOT，来源于脐带血单个核细胞，其特征是具有FOXP3(+)CD4(+)CD8(+)CD25（+）表型的人类细胞毒性调节性T （Treg）细胞系。在这里，我们描述了HOZOT细胞的一个新特性：它们主动渗透到各种人类癌细胞系中，但不渗透到正常细胞系中，并形成明显的细胞中细胞结构。在细胞渗透过程中，我们观察到附着在靶细胞上的HOZOT细胞似乎首先将细胞核插入靶细胞的细胞质中，这与吞噬过程不同。此外，阻断实验表明，主要组织相容性复合体I类是HOZOT细胞的靶细胞识别分子之一。此外，我们提出HOZOT细胞与靶癌细胞之间的细胞内结构可能是HOZOT细胞的细胞毒性机制之一。
We previously established a novel cell line, termed HOZOT, derived from umbilical cord blood mononuclear cells that is characterized as a human cytotoxic regulatory T (Treg) cell line with a FOXP3(+)CD4(+)CD8(+)CD25(+) phenotype. Here, we describe a new property of HOZOT cells: they actively penetrate into a variety of human cancer cell lines, but not into normal cell lines, and form apparent cell-in-cell structures. In the process of cell penetration, we observed that HOZOT cells adhered to target cells seemed to first insert their nuclei into the cytoplasm of target cells, distinct from the process of phagocytosis. In addition, blocking experiments showed that major histocompatibility complex class I is one of the target cell recognition molecules for HOZOT cells. Furthermore, we propose that cell-in-cell structures between HOZOT cells and target cancer cells could be one of the cytotoxic mechanisms of HOZOT cells.