The expression strategy of goose parvovirus exhibits features of both the Dependovirus and Parvovirus genera
The expression strategy of goose parvovirus exhibits features of both the Dependovirus and Parvovirus genera
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DOI：
10.1128/jvi.79.17.11035-11044.2005
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发表时间：
2005-09-01
影响因子：
5.4
通讯作者：
Pintel, D
中科院分区：
文献类型：
--
作者：
Qiu, JM;Cheng, F;Pintel, D
鹅细小病毒（GPV）的RNA转录谱被确定，并且它是细小病毒科的细小病毒亚科的细小病毒属和依赖病毒属的特征的令人惊讶的杂交体。与5型依赖病毒腺相关病毒类似，从GPV上游P9启动子转录的RNA（编码病毒非结构蛋白）在位于基因组中心内含子内的多聚腺苷酸化位点[（pA）p]处高效多聚腺苷酸化。（PA）p的有效利用需要与中央内含子的A2 3'剪接位点的多聚嘧啶段重叠的下游元件。还确定了有效利用（PA）P所需的上游元素。从P42启动子转录的RNA，假定编码病毒衣壳蛋白，主要通过（pA）p延伸，并在位于基因组右端的位点（pA）d处聚腺苷酸化，并最终以高效率剪接。没有检测到与依赖病毒P19启动子类似的启动子;然而，与小鼠和细小病毒属其他成员的微小病毒相似，P9启动子产生的前mRNA的显著部分在推定的GPV Rep 1编码区内额外剪接，并可能编码额外的、较小的非结构蛋白。与细小病毒属成员相似，GPV P42启动子的可检测活性高度依赖于GPV Rep 1蛋白的反式激活，其方式依赖于与位于P42启动子中的顺式元件结合。
The RNA transcription profile of the goose parvovirus (GPV) was determined, and it is a surprising hybrid of features of the Parvovirus and Dependovirus genera of the Parvovirinae subfamily of the Parvoviridae. Similar to the Dependovirus adeno-associated virus type 5, RNAs transcribed from the GPV upstream P9 promoter, which encode the viral nonstructural proteins, were polyadenylated at a high efficiency at a polyadenylation site [(pA)p] located within an intron in the center of the genome. Efficient usage of (pA)p required a downstream element that overlaps with the polypyrimidine tract of the A2 3' splice site of the central intron. An upstream element required for efficient use of (pA)p was also identified. RNAs transcribed from the P42 promoter, presumed to encode the viral capsid proteins, primarily extended through (pA)p and were polyadenylated at a site, (pA)d, located at the right end of the genome and ultimately spliced at a high efficiency. No promoter analogous to the Dependovirus P19 promoter was detected; however, similar to minute virus of mice and other members of the Parvovirus genus, a significant portion of pre-mRNAs generated from the P9 promoter were additionally spliced within the putative GPV Rep1 coding region and likely encode an additional, smaller, nonstructural protein. Also similar to members of the Parvovirus genus, detectable activity of the GPV P42 promoter was highly dependent on transactivation by the GPV Rep1 protein in a manner dependent on binding to a cis-element located in the P42 promoter.