Transgenic Citrus Expressing an Arabidopsis NPR1 Gene Exhibit Enhanced Resistance against Huanglongbing (HLB; Citrus Greening).
Transgenic Citrus Expressing an Arabidopsis NPR1 Gene Exhibit Enhanced Resistance against Huanglongbing (HLB; Citrus Greening).
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DOI：
10.1371/journal.pone.0137134
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Grosser J
中科院分区：
文献类型：
--
作者：
Dutt M;Barthe G;Irey M;Grosser J
商业甜橙子品种缺乏对黄龙病（HLB）的抗性，黄龙病是一种严重的韧皮部限制性细菌疾病，通常是致命的。为了发展对HLB的持续抗病性，产生了表达在组成型CaMV 35 S启动子或韧皮部特异性拟南芥SUC 2（AtSUC 2）启动子控制下的拟南芥NPR 1基因的转基因甜橙子栽培品种“Hamlin”和“瓦伦西亚”。AtNPR 1的过表达导致具有正常表型的树木表现出增强的HLB抗性。韧皮部特异性表达NPR 1对提高抗病性同样有效。转基因树表现出降低疾病的严重程度和几行仍然无病，即使在36个月的种植在高疾病压力现场。NPR 1基因的表达诱导了参与植物防御信号传导途径的几个天然基因的表达。植物来源的AtNPR 1基因可以作为开发全植物T-DNA来源的消费者友好型转基因树的组成部分。
Commercial sweet orange cultivars lack resistance to Huanglongbing (HLB), a serious phloem limited bacterial disease that is usually fatal. In order to develop sustained disease resistance to HLB, transgenic sweet orange cultivars ‘Hamlin’ and ‘Valencia’ expressing an Arabidopsis thaliana NPR1 gene under the control of a constitutive CaMV 35S promoter or a phloem specific Arabidopsis SUC2 (AtSUC2) promoter were produced. Overexpression of AtNPR1 resulted in trees with normal phenotypes that exhibited enhanced resistance to HLB. Phloem specific expression of NPR1 was equally effective for enhancing disease resistance. Transgenic trees exhibited reduced diseased severity and a few lines remained disease-free even after 36 months of planting in a high-disease pressure field site. Expression of the NPR1 gene induced expression of several native genes involved in the plant defense signaling pathways. The AtNPR1 gene being plant derived can serve as a component for the development of an all plant T-DNA derived consumer friendly GM tree.