In vivo properties of cerebellar interneurons in the macaque caudal vestibular vermis.
In vivo properties of cerebellar interneurons in the macaque caudal vestibular vermis.
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猕猴尾部前庭蚓部小脑中间神经元的体内特性。
DOI：
10.1113/jphysiol.2014.278523
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Angelaki,DoraE
中科院分区：
文献类型：
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作者：
Meng,Hui;Laurens,Jean;Blázquez,PabloM;Angelaki,DoraE
关键点我们量化了X小叶（结节）和IXc，d中中间神经元的自发和刺激驱动反应基于基线放电，至少可以区分三种类型的神经元群体。第一，存在一组具有高平均放电率的非常规则的放电神经元。第二，有一组低放电神经元，具有一定的放电规律。第三，我们还发现了假定的中间神经元，其放电规律和平均放电率与生理学上鉴定的浦肯野细胞的放电规律和平均放电率难以区分。苔藓纤维具有未加工的耳石样传入特性。摘要小脑皮质是大脑中研究最多的回路之一，包括不同类别的兴奋性和抑制性中间神经元。一些研究试图描述小脑中间神经元的体内特性，但目前对其刺激驱动特性知之甚少。在这里，我们量化了前庭刺激期间猕猴尾蚓部小叶X（结节）和IXc，d（腹侧悬雍垂）中中间神经元的自发和刺激驱动反应。中间神经元被鉴定为距离浦肯野细胞层>100 μm的细胞，其不表现出复杂的尖峰。基于基线放电，可以区分三种类型的中间神经元。首先，有一组非常有规律的中间神经元具有高的平均放电率，这些中间神经元始终编码倾斜，而不是平移头部运动。第二，有一组低放电的中间神经元，具有一定的放电规律。这一组具有更多样化的前庭特性，其中大多数是平移选择性的，少数是倾斜或重力惯性加速度选择性的。第三，我们也遇到了类似于浦肯野细胞放电规律和平均放电率的中间神经元。该小组还编码倾斜和平移信号的混合物。一些苔藓纤维显示出未加工的耳石传入特性，编码重力惯性加速度。我们的结论是，倾斜和翻译选择性信号，反映了神经计算转换前庭传入信息，不仅遇到浦肯野细胞反应。相反，小脑皮质内的上游中间神经元也具有类似的特性，因此意味着广泛的网络计算。
Key pointsWe quantify both spontaneous and stimulus‐driven responses of interneurons in lobules X (nodulus) and IXc,d (ventral uvula) of the caudal vermis during vestibular stimulation.Based on baseline firing, at least three types of neuronal populations could be distinguished.First, there was a group of very regular firing neurons with high mean discharge rates.Second, there was a group of low firing neurons with a range of discharge regularity.Third, we also encountered putative interneurons with discharge regularity and mean firing rates that were indistinguishable from those of physiologically identified Purkinje cells.The vestibular responses of putative interneurons were generally similar to those of Purkinje cells, thus encoding tilt, translation or mixtures of these signals.Mossy fibres showed unprocessed, otolith afferent‐like properties.AbstractThe cerebellar cortex is among the brain's most well‐studied circuits and includes distinct classes of excitatory and inhibitory interneurons. Several studies have attempted to characterize thein vivoproperties of cerebellar interneurons, yet little is currently known about their stimulus‐driven properties. Here we quantify both spontaneous and stimulus‐driven responses of interneurons in lobules X (nodulus) and IXc,d (ventral uvula) of the macaque caudal vermis during vestibular stimulation. Interneurons were identified as cells located >100 μm from the Purkinje cell layer that did not exhibit complex spikes. Based on baseline firing, three types of interneurons could be distinguished. First, there was a group of very regular firing interneurons with high mean discharge rates, which consistently encoded tilt, rather than translational head movements. Second, there was a group of low firing interneurons with a range of discharge regularity. This group had more diverse vestibular properties, where most were translation‐selective and a few tilt‐ or gravitoinertial acceleration‐selective. Third, we also encountered interneurons that were similar to Purkinje cells in terms of discharge regularity and mean firing rate. This group also encoded mixtures of tilt and translation signals. A few mossy fibres showed unprocessed, otolith afferent‐like properties, encoding the gravitoinertial acceleration. We conclude that tilt‐ and translation‐selective signals, which reflect neural computations transforming vestibular afferent information, are not only encountered in Purkinje cell responses. Instead, upstream interneurons within the cerebellar cortex are also characterized by similar properties, thus implying a widespread network computation.