RAPID ELECTROPHORETIC PROCEDURE FOR DETECTION OF SDS-RELEASED ONCORNA-VIRAL RNA USING POLYACRYLAMIDE-AGAROSE GELS
RAPID ELECTROPHORETIC PROCEDURE FOR DETECTION OF SDS-RELEASED ONCORNA-VIRAL RNA USING POLYACRYLAMIDE-AGAROSE GELS
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DOI：
10.1016/0003-2697(73)90146-2
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发表时间：
1973-01-01
影响因子：
2.9
通讯作者：
WOLFF, JS
中科院分区：
文献类型：
--
作者：
HAREWOOD, K;WOLFF, JS
本文介绍了一种在丙烯酰胺琼脂糖凝胶上快速分析十二烷基硫酸钠释放的病毒RNA的方法。利用该方法对Rauscher白血病病毒和Mason-Pfizer猴病毒进行的研究表明，苯酚提取和十二烷基硫酸钠释放的病毒RNA的RNA组分的分布和回收类似。该方法的灵敏度约为蔗糖梯度分析的20倍，可用于病毒浓度(EM颗粒计数或放射性)较低的情况。给出的数据说明了该方法的通用性。
A rapid method for analyzing SDS-released viral RNA on acrylamideagarose gels is described. Studies carried out on Rauscher leukemia virus and Mason-Pfizer monkey virus utilizing this method show that the distribution and recovery of the RNA components are similar for phenol-extracted or SDS-released viral RNA. The method is approximately 20 times more sensitive than sucrose gradient analysis and can be utilized in cases where virus concentration (EM particle count or radioactivity) is low. Data presented illustrate the versatility of this method.