MAPPING OF THE DISTRIBUTION OF POLYSIALYLATED NEURAL CELL-ADHESION MOLECULE THROUGHOUT THE CENTRAL-NERVOUS-SYSTEM OF THE ADULT-RAT - AN IMMUNOHISTOCHEMICAL STUDY
MAPPING OF THE DISTRIBUTION OF POLYSIALYLATED NEURAL CELL-ADHESION MOLECULE THROUGHOUT THE CENTRAL-NERVOUS-SYSTEM OF THE ADULT-RAT - AN IMMUNOHISTOCHEMICAL STUDY
复制标题
DOI：
10.1016/0306-4522(92)90107-d
复制
发表时间：
1992-07-01
期刊：
影响因子：
3.3
通讯作者：
THEODOSIS, DT
中科院分区：
文献类型：
--
作者：
BONFANTI, L;OLIVE, S;THEODOSIS, DT
在神经系统中，神经细胞粘附分子在发育过程中在细胞表面发生变化，从高度富集聚唾液酸残基的形式变为含有少得多的唾液酸的几种同种型，并且被认为参与神经元组的结构化和神经元连接的建立。然而，最近的观察表明，它可能不限于发育中的组织，因为它仍然存在于某些成年神经元中心，可以进行形态重组。因此，在这项研究中，我们系统地研究了多聚唾液酸化的神经细胞粘附分子免疫反应性在整个成年雄性和雌性大鼠中枢神经系统的分布，使用战斗显微镜免疫细胞化学和免疫印迹分析与抗体，特异性地识别该分子的多聚唾液酸残基。同时，我们比较了这种免疫反应性，由于所有亚型的神经细胞粘附分子，检测与多克隆血清提出了对NH 2-terminal的蛋白质。免疫反应性由于聚唾液酸化亚型一致可视化在几个离散的地区的成年人大脑和脊髓。细胞间点状免疫标记的特征是在某些下丘脑和丘脑核、脊髓背角浅层、海马齿状回腹侧部分、外侧膝状体、臂旁核和缰核、终纹床核、中脑中央灰质和嗅球中染色。在其他区域，如梨状皮质，背侧的齿状回和海马伞和X层的脊髓，分离的神经元样细胞完全充满免疫标记或显示出表面反应的细胞体和过程。在中央管和侧脑室的室管膜层内，有时在脊髓的外周白色物质中也观察到高度免疫反应性的孤立胶质样细胞。与此不同的是，由于神经细胞粘附分子的所有亚型的染色是广泛和弥漫性的整个大脑和脊髓。免疫印迹分析证实了在视上核和海马中的多唾液酸化亚型的表达，它与弱唾液酸化亚型一起发生。没有明显的差异，检测到的免疫反应性的数量或分布，由于多聚唾液酸化亚型的性别或年龄的动物（3和12个月的年龄之间）。因此，我们的研究表明，明确的区域的中枢神经系统的成年大鼠继续表达的神经细胞粘附分子的多聚唾液酸化亚型。由于许多这些领域是已知的，在特定的生理和实验条件下进行结构重组，我们的观察支持的假设，聚唾液酸化可能是一个关键因素，让某些神经元和神经胶质细胞，以体现其能力的结构可塑性在成年期。
In the nervous system, the neural cell adhesion molecule changes at the cell surface during development, from a form highly enriched in polysialic residues to several isoforms containing much less sialic acid, and is thought to participate in the structuring of neuronal groups and in the establishment of neuronal connections. Recent observations have indicated, however, that it may not be restricted to developing tissues since it is still present in certain adult neuronal centres which can undergo morphological reorganization. In this study, therefore, we examined systematically the distribution of polysialylated neural cell adhesion molecule immunoreactivity throughout the central nervous system of adult male and female rats, using fight microscopic immunocytochemistry and immunoblot analysis with an antibody that specifically recognizes the polysialic residues of the molecule. Concomitantly, we compared this immunoreactivity to that due to all isoforms of the neural cell adhesion molecule, detected with a polyclonal serum raised against the NH2-terminal of the protein.Immunoreactivity due to the polysialylated isoform was consistently visualized in several discrete areas of the adult brain and spinal cord. An intercellular punctate immunolabelling characterized the staining in certain hypothalamic and thalamic nuclei, superficial laminae of the dorsal horn of the spinal cord, ventral portion of the dentate gyrus of the hippocampus, lateral geniculate, parabrachial and habenular nuclei, bed nucleus of the stria terminalis, mesencephalic central gray and olfactory bulb. In other areas, such as the piriform cortex, dorsal aspect of the dentate gyrus and fimbria and lamina X of the spinal cord, isolated neuronal-like cells were either completely filled with immunolabel or showed a surface reaction on their cell bodies and processes. Highly immunoreactive isolated glial-like cells were also noted within the ependymal layer of the central canal and lateral ventricles and at times in the peripheral white matter of the spinal cord. In contrast to this discrete localization, staining due to all isoforms of the neural cell adhesion molecule was widespread and diffuse throughout the brain and spinal cord.The expression of the polysialylated isoform in the supraoptic nucleus and hippocampus was confirmed by immunoblot analysis; it occurred together with weakly sialylated isoforms. No obvious differences were detected in the amount or distribution of immunoreactivity due to the polysialylated isoform in relation to the sex or age of the animals (between three and 12 months of age).Our study thus demonstrates that well-defined areas of the central nervous system of the adult rat continue to express the polysialylated isoform of the neural cell adhesion molecule. Since many of these areas are known to undergo structural reorganization under particular physiological and experimental conditions, our observations support the hypothesis that polysialylation may be a crucial factor in allowing certain neurons and glial cells to manifest their capacity for structural plasticity in adulthood.