Tangluoning, a traditional Chinese medicine, attenuates in vivo and in vitro diabetic peripheral neuropathy through modulation of PERK/Nrf2 pathway
Tangluoning, a traditional Chinese medicine, attenuates in vivo and in vitro diabetic peripheral neuropathy through modulation of PERK/Nrf2 pathway
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糖络宁是一种中药，通过调节 PERK/Nrf2 通路减轻体内和体外糖尿病周围神经病变，
DOI：
10.1038/s41598-017-00936-9
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发表时间：
2017-04-21
影响因子：
4.6
通讯作者：
Xu, Liping
中科院分区：
文献类型：
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作者：
Yang, Xinwei;Yao, Weijie;Xu, Liping
长期高血糖诱导的氧化应激和内质网应激已被证明在糖尿病周围神经病变（DPN）的进展中起关键作用。PERK/Nrf 2通路在与细胞存活相关的氧化和内质网（ER）应激中起主导作用。本研究探讨中药糖络宁（TLN）对链脲佐菌素诱导的DPN大鼠模型PERK/Nrf 2通路和细胞凋亡的调节作用，以及血清TLN对150 mM葡萄糖培养的Schwann细胞PERK/Nrf 2通路、细胞凋亡、细胞内活性氧和线粒体膜电位的影响。在体内和体外实验DPN模型中，TLN通过上调p-PERK、Nrf 2/ARE通路和下调CHOP相关的凋亡通路，通过PERK/Nrf 2通路减轻氧化和ER应激以及凋亡。
Prolonged hyperglycemia-induced oxidative stress and endoplasmic reticulum stress have been demonstrated to play a key role in progression of diabetic peripheral neuropathy (DPN). PERK/Nrf2 pathway plays a predominant role in oxidative and endoplasmic reticulum (ER) stress which is associated with cell survival. This study examined the modulation of the PERK/Nrf2 pathway and apoptosis by a traditional Chinese medicine Tangluoning (TLN) in streptozotocin-induced DPN rat models and the effects of serum TLN on the PERK/Nrf2 pathway, apoptosis, intracellular reactive oxygen species and mitochondrial membrane potential in Schwann cells cultured in 150 mM glucose. It is found that TLN attenuated oxidative and ER stress and apoptosis through the PERK/Nrf2 pathway by upregulating p-PERK, Nrf2/ARE pathways and downregulating the CHOP-related apoptosis pathways in the experimental DPN models both in vivo and in vitro.