Vitronectin expression and interaction with receptors in smooth muscle cells from human atheromatous plaque
Vitronectin expression and interaction with receptors in smooth muscle cells from human atheromatous plaque
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DOI：
10.1161/01.atv.18.2.168
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发表时间：
1998-02-01
影响因子：
8.7
通讯作者：
Bonnet, J
中科院分区：
文献类型：
--
作者：
Dufourcq, P;Louis, H;Bonnet, J
玻连蛋白（VN）是一种血浆糖蛋白，可促进细胞粘附并诱导培养的人平滑肌细胞（SMC）迁移。VN在人动脉粥样硬化斑块中有一定的蓄积，但其起源和作用尚不明确。本实验采用原位杂交和免疫组织化学方法，对15例颈动脉粥样硬化斑块标本进行了VN合成及其与α v β 3和α v β 5受体共定位的研究。在血管内膜和中膜中观察到VN蛋白和mRNA的强表达，在内膜中，VN mRNA的表达与SMC共定位，表明这些细胞产生VN，这可能是其在动脉粥样硬化斑块中积聚的原因。在培养的SMC中，免疫沉淀改变代谢标记证实人SMC确实合成玻连蛋白。共聚焦显微镜检查显示，VN与其受体α v β 3和α v β 5共定位于动脉粥样硬化内膜中，但与VN接触的培养物中SMC上VN受体的分布不同。，这些观察！提示VN通过不同的SMC膜受体参与动脉粥样硬化的形成。
Vitronectin (VN) is a plasma glycoprotein that promotes cell attachment and induces migration of human smooth muscle cells (SMCs) in culture. VN has been observed to accumulate in human atherosclerotic plaques, although its origin and role in atherosclerosis are not yet established, In the present experiments, synthesis of VN by intimal cells and its colocalization with receptors, alpha v beta 3 and alpha v beta 5, were studied by in situ hybridization and immunohistochemistry on 15 human atherosclerotic plaques ii-om carotid arteries obtained after surgery. Strong VN protein and mRNA expression was observed in the intima and in the media, In the intima, VN mRNA expression was colocalized with SMCs, indicating that these cells produce VN, which may account for its accumulation in atherosclerotic plaques, In SMCs in culture, immunoprecipitation alter metabolic labeling demonstrated that human SMCs do synthesize vitronectin. Confocal microscopic examination showed that VN colocalized with its receptors, alpha v beta 3 and alpha v beta 5, in the atherosclerotic intima, However, the distribution of the VN receptors on SMCs in culture in contact with VN was different., These observations! suggest that VN plays various parts in atherogenesis via different SMC membrane receptors.