PgMYB2, a MeJA-Responsive Transcription Factor, Positively Regulates the Dammarenediol Synthase Gene Expression in Panax Ginseng
PgMYB2, a MeJA-Responsive Transcription Factor, Positively Regulates the Dammarenediol Synthase Gene Expression in Panax Ginseng
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PgMYB2 是一种 MeJA 响应转录因子，可正向调节人参中达玛烯二醇合酶基因的表达
DOI：
10.3390/ijms20092219
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发表时间：
2019-05-01
影响因子：
5.6
通讯作者：
Luo, Zhiyong
中科院分区：
文献类型：
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作者：
Liu, Tuo;Luo, Tiao;Luo, Zhiyong
MYB转录因子家族成员在植物生长调节、防御反应和次生代谢中发挥着不同的作用。然而，MYB基因在人参中的表达还未见报道。在这项研究中，我们从人参不定根，PgMYB 2，它编码一个R2 R3-MYB蛋白的基因。亚细胞定位结果表明，PgMYB 2蛋白仅在洋葱表皮细胞核中表达。PgMYB 2在人参根中的表达量最高，且受植物激素茉莉酸甲酯（MeJA）的显著诱导。此外，PgMYB 2蛋白与达玛烯二醇合成酶（DDS）的启动子之间的结合相互作用被发现在酵母菌株Y1 H黄金。电泳迁移率变动分析（EMSA）确定了PgMYB 2与PgDDS相互作用的结合位点，瞬时过表达PgMYB 2的结果也表明PgMYB 2可能正调控PgDDS的表达。基于PgDDS基因在人参皂苷合成中的关键作用，有理由相信本研究将有助于进一步研究人参中MYB家族，并最终通过遗传和代谢工程提高人参皂苷的产量。
The MYB transcription factor family members have been reported to play different roles in plant growth regulation, defense response, and secondary metabolism. However, MYB gene expression has not been reported in Panax ginseng. In this study, we isolated a gene from ginseng adventitious root, PgMYB2, which encodes an R2R3-MYB protein. Subcellular localization revealed that PgMYB2 protein was exclusively detected in the nucleus of Allium cepa epidermis. The highest expression level of PgMYB2 was found in ginseng root and it was significantly induced by plant hormones methyl jasmonate (MeJA). Furthermore, the binding interaction between PgMYB2 protein and the promoter of dammarenediol synthase (DDS) was found in the yeast strain Y1H Gold. Moreover, the electrophoretic mobility shift assay (EMSA) identified the binding site of the interaction and the results of transiently overexpressing PgMYB2 in plants also illustrated that it may positively regulate the expression of PgDDS. Based on the key role of PgDDS gene in ginsenoside synthesis, it is reasonable to believe that this report will be helpful for the future studies on the MYB family in P. ginseng and ultimately improving the ginsenoside production through genetic and metabolic engineering.