Duplication of a manganese/cadmium transporter gene reduces cadmium accumulation in rice grain
Duplication of a manganese/cadmium transporter gene reduces cadmium accumulation in rice grain
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DOI：
10.1038/s43016-022-00569-w
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发表时间：
2022-08
期刊：
影响因子：
23.2
通讯作者：
En Yu;Wenguang Wang;N. Yamaji;S. Fukuoka;Jing Che;D. Ueno;T. Ando;Fenglin Deng;K. Hori;M. Yano;R. Shen;J. Ma
中科院分区：
文献类型：
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作者：
En Yu;Wenguang Wang;N. Yamaji;S. Fukuoka;Jing Che;D. Ueno;T. Ando;Fenglin Deng;K. Hori;M. Yano;R. Shen;J. Ma
全球土壤被有毒镉 (Cd) 污染是一个严重的健康威胁。在这里，我们发现编码锰/镉转运蛋白的基因 OsNramp5 的串联重复导致了老稻品种 Pokkali 的低镉积累。这种重复使 OsNramp5 基因的表达加倍，但没有改变其空间表达模式和细胞定位。 OsNramp5 的较高表达增加了根细胞对 Cd 和 Mn 的吸收，但减少了 Cd 向木质部的释放。当在镉严重污染的土壤中种植时，通过回交将该等位基因引入到优良水稻品种越光中，显着降低了谷物中镉的积累，但不影响谷物产量和食用品质。该研究不仅揭示了低Cd积累的分子机制，而且为选育低Cd积累的水稻品种提供了有用的靶标。
Global contamination of soils with toxic cadmium (Cd) is a serious health threat. Here we found that a tandem duplication of a gene encoding a manganese/Cd transporter,OsNramp5, was responsible for low-Cd accumulation in Pokkali, an old rice cultivar. This duplication doubled the expression ofOsNramp5gene but did not alter its spatial expression pattern and cellular localization. Higher expression ofOsNramp5increased uptake of Cd and Mn into the root cells but decreased Cd release to the xylem. Introgression of this allele into Koshihikari, an elite rice cultivar, through backcrossing significantly reduced Cd accumulation in the grain when cultivated in soil heavily contaminated with Cd but did not affect both grain yield and eating quality. This study not only reveals the molecular mechanism underlying low-Cd accumulation but also provides a useful target for breeding rice cultivars with low-Cd accumulation.