Evidence for rhombomeric organization of multiple respiratory oscillators in the bullfrog brainstem
Evidence for rhombomeric organization of multiple respiratory oscillators in the bullfrog brainstem
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DOI：
10.1016/j.resp.2012.12.007
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发表时间：
2013-03-01
影响因子：
2.3
通讯作者：
Hedrick, Michael S.
中科院分区：
文献类型：
--
作者：
Klingler, Michael J.;Hedrick, Michael S.
无尾猿的脑干以节段组织成菱形小体(r)，这种节段组织在整个发育过程中被独特地保存下来。我们假设与脑神经（CN）相关的菱球也包含能够单独产生有节奏运动输出（爆发）的振荡子。我们使用牛蛙（Lithobates catesbeianus）变质前和变质后的体外脑干制剂来确定振荡体的双形组织是否存在于整个发育过程中。将脑干横切成含有一个或多个菱形球的节段，用附着在cnv、X和XII上的吸电极测量运动输出。85%的蝌蚪和91%的青蛙在前节（r0-r5）， 27%的蝌蚪和18%的青蛙在中节（r6-r7）， 77%的蝌蚪和55%的青蛙在尾节（r8）观察到节律性运动输出。横断后，破裂频率和整个神经振幅显著降低。这些数据支持了一种假设，即在无尾动物的整个发育过程中，脑干振荡与特定的菱形体群有关。(C) 2012 Elsevier B.V.版权所有
The anuran brainstem is segmentally organized into rhombomeres (r) and this segmental organization is uniquely preserved throughout development. We hypothesized that rhombomeres associated with cranial nerves (CN) also contain oscillators that are capable of producing rhythmic motor output (bursts) in isolation. We used in vitro brainstem preparations from pre- and post-metamorphic bullfrogs (Lithobates catesbeianus) to determine if rhombomeric organization of oscillators is present throughout development. Brainstems were transected into segments containing one or more rhombomeres and motor output was measured with suction electrodes attached to CN V, X and XII. Rhythmic motor output was observed in 85% of tadpoles and 91% of frogs in an anterior segment (r0-r5), 27% of tadpoles and 18% of frogs in the middle segment (r6-r7), and 77% of tadpoles and 55% of frogs in the caudal segment (r8). There were significant reductions in burst frequency and whole nerve amplitude following transections. These data support the hypothesis that brainstem oscillators associated with specific groups of rhombomeres are present throughout development in anurans. (C) 2012 Elsevier B.V. All rights reserved.