Minimal Model of Plasma Membrane Heterogeneity Requires Coupling Cortical Actin to Criticality
Minimal Model of Plasma Membrane Heterogeneity Requires Coupling Cortical Actin to Criticality
复制标题
DOI：
10.1016/j.bpj.2011.02.029
复制
发表时间：
2011-04-06
影响因子：
3.4
通讯作者：
Veatch, Sarah L.
中科院分区：
文献类型：
--
作者：
Machta, Benjamin B.;Papanikolaou, Stefanos;Veatch, Sarah L.
我们提出了一个最小的质膜异质性模型，结合关键性与皮层细胞骨架的连接。这个模型的发展是由最近观察到的细胞膜囊泡脱离皮层细胞骨架的微米级临界波动的动机。我们将关键性使用一个保守的顺序参数伊辛模型耦合到一个简单的肌动蛋白细胞骨架相互作用，通过点样钉扎位点。使用这个最小的模型，我们概括了几个实验观察质膜筏异质性。在生理温度下的小（r类似于20 nm）和动态波动由临界性引起。包括与皮层细胞骨架的连接性破坏了大的波动，防止了低温下的宏观相分离（T
We present a minimal model of plasma membrane heterogeneity that combines criticality with connectivity to cortical cytoskeleton. The development of this model was motivated by recent observations of micron-sized critical fluctuations in plasma membrane vesicles that are detached from their cortical cytoskeleton. We incorporate criticality using a conserved order parameter Ising model coupled to a simple actin cytoskeleton interacting through point-like pinning sites. Using this minimal model, we recapitulate several experimental observations of plasma membrane raft heterogeneity. Small (r similar to 20 nm) and dynamic fluctuations at physiological temperatures arise from criticality. Including connectivity to the cortical cytoskeleton disrupts large fluctuations, prevents macroscopic phase separation at low temperatures (T