Notch1 signaling is activated in cells expressing embryonic stem cell proteins in human primary nasopharyngeal carcinoma.
Notch1 signaling is activated in cells expressing embryonic stem cell proteins in human primary nasopharyngeal carcinoma.
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DOI：
10.2310/7070.2009.090150
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发表时间：
2010-04
期刊：
影响因子：
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通讯作者：
Yue Zhang;Jianhua Peng;Huxiang Zhang;Yi Zhu;Li Wan;Jianfu Chen;Xiaoyun Chen;Renyu Lin;He Li;X. Mao;K. Jin
中科院分区：
文献类型：
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作者：
Yue Zhang;Jianhua Peng;Huxiang Zhang;Yi Zhu;Li Wan;Jianfu Chen;Xiaoyun Chen;Renyu Lin;He Li;X. Mao;K. Jin
目的探讨Notch 1信号通路在鼻咽癌中的表达。方法：我们用抗胚胎干细胞（ES）蛋白和抗Notch1信号成分的抗体对手术切除的鼻咽癌进行免疫细胞化学。结果发现ES细胞蛋白标志物SOX 2和OCT 4在三种亚型NPC的细胞亚群中均有表达，而在正常对照组中几乎没有表达。免疫双标显示SOX 2和OCT 4阳性细胞共表达增殖标志物，提示人NPC中可能含有癌干细胞样细胞。此外，我们发现Notch1信号在NPC中被激活。共聚焦图像显示，Notch1信号激活形式和Hes1（Notch1信号的下游靶点）主要存在于SOX 2和OCT 4阳性细胞中。结论Notch 1信号通路可能是鼻咽癌肿瘤干细胞样细胞的调控因子。
OBJECTIVE To explore the expression of Notch1 signaling pathway in nasopharyngeal carcinoma (NPC). METHODS We performed immunocytochemistry on surgically resected NPC using antibodies against embryonic stem (ES) cell proteins and against Notch1 signaling components. RESULTS We found that ES cell protein markers SOX2 and OCT4 were expressed in a subpopulation of cells for all three subtypes of NPC but barely in the normal control. Double immunostaining shows that SOX2- and OCT4-positive cells coexpressed proliferative markers, suggesting that human NPC may contain cancer stem-like cells. In addition, we found that Notch1 signaling was activated in NPC. Confocal images show that the Notch1 signaling activated form and Hes1, a downstream target of Notch1 signaling, was predominantly found in SOX2- and OCT4-positive cells. CONCLUSION Our findings suggest that the Notch1 signaling pathway might be a regulator of cancer stem-like cells in NPC.