Membrane properties of plant sterols in phospholipid bilayers as determined by differential scanning calorimetry, resonance energy transfer and detergent-induced solubilization
Membrane properties of plant sterols in phospholipid bilayers as determined by differential scanning calorimetry, resonance energy transfer and detergent-induced solubilization
复制标题
DOI：
10.1016/j.bbamem.2004.05.006
复制
发表时间：
2004-08-30
影响因子：
3.4
通讯作者：
Slotte, JP
中科院分区：
文献类型：
--
作者：
Halling, KK;Slotte, JP
植物甾醇作为降胆固醇剂的使用越来越多，这使得进一步研究植物甾醇在膜中的可能作用成为必要。本研究采用差示扫描量热法（DSC）、反式帕里纳酸（tPA）与2-羟基丁酸（2-HT）之间的共振能量转移（RET）、红外光谱（IR）和透射电镜（TEM）研究了胆固醇、菜油甾醇、β-谷甾醇和豆甾醇对磷脂双分子膜的影响。（6-（7-硝基苯并-2-氧杂-1，3-二唑-4-基）氨基）己酰基-1-十六酰基-sn-甘油基-3-磷酸胆碱（NBD-PC）和Triton X-100诱导的增溶作用。使用的磷脂为1，2-二棕榈酰-sn-甘油基-3-磷酸胆碱（DPPC）、D-双-N-棕榈酰-鞘氨醇（PSM）和1-棕榈酰-2-油酰-sn-甘油基-3-磷酸胆碱（POPC）。在DSC实验中，它表明，甾醇的不同，在他们的影响上的熔融温度的甾醇贫和甾醇丰富的领域在DPPC和PSM双层。与胆固醇相比，植物甾醇引起两种转变的温度较低。植物甾醇也导致较低的转变温度，与胆固醇相比，含甾醇的DPPC和PSM双层进行了研究RET。在洗涤剂溶解实验中，Triton X-100和POPC之间的总摩尔比在溶解的开始（R-t，R-sat）是较高的双层含有植物甾醇，与膜含有胆固醇。总之，本研究中提出的观察结果表明，菜油甾醇，β-谷甾醇和豆甾醇的相互作用不如胆固醇与磷脂，导致其域性质的可测量的差异。(C)2004 Elsevier B. V.保留所有权利。
The increased use of plant sterols as cholesterol-lowering agents warrants further research on the possible effects of plant sterols in membranes. In this study, the effects of the incorporation of cholesterol, campesterol, beta-sitosterol and stigmasterol in phospholipid bilayers were investigated by differential scanning calorimetry (DSC), resonance energy transfer (RET) between trans parinaric acid (tPA) and 2-(6(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl-1-hexadecanoyl-sn-glycero-3-phosphocholine (NBD-PC), and Triton X-100-induced solubilization. The phospholipids used were 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), D-erythro-N-palmitoyl-sphingomyclin (PSM), and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). In DSC experiments, it was demonstrated that the sterols differed in their effect on the melting temperatures of both the sterol-poor and the sterol-rich domains in DPPC and PSM bilayers. The plant sterols gave rise to lower temperatures of both transitions, when compared with cholesterol. The plant sterols also resulted in lower transition temperatures, in comparison with cholesterol, when sterol-containing DPPC and PSM bilayers were investigated by RET. In the detergent solubilization experiments, the total molar ratio between Triton X-100 and POPC at the onset of solubilization (R-t,R-sat) was higher for bilayers containing plant sterols, in comparison with membranes containing cholesterol. Taken together, the observations presented in this study indicate that campesterol, beta-sitosterol and stigmasterol interacted less favorably than cholesterol with the phospholipids, leading to measurable differences in their domain properties. (C) 2004 Elsevier B.V. All rights reserved.