Transport of a hydrophobic biosynthetic precursor by lipophorin in the hemolymph of a geometrid female moth which secretes an epoxyalkenyl sex pheromone
Transport of a hydrophobic biosynthetic precursor by lipophorin in the hemolymph of a geometrid female moth which secretes an epoxyalkenyl sex pheromone
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DOI：
10.1016/j.ibmb.2006.04.006
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发表时间：
2006-07-01
影响因子：
3.8
通讯作者：
Ando, Tetsu
中科院分区：
文献类型：
--
作者：
Matsuoka, Kanae;Tabunoki, Hiroko;Ando, Tetsu
先前对一种几何物种Ascotis selenaria白垩纪的实验表明，信息素腺体外产生的相应的3,6,9-三烯生物合成了信息素c - 19,3,4 -环氧-6,9-二烯，并与脂蛋白结合后通过血淋巴运输到它。为了阐明这种转运，我们用KBr平衡密度梯度超离心纯化了在SDS-PAGE上显示两个条带（apoLp I约为250kDa和apoLp II约为80kDa）的雌性飞蛾高密度脂蛋白（HDLp），并通过溶剂提取物的GC-MS分析证实了其与三烯的关联。接下来，HDLp的作用揭示了局部应用氘化三烯基前体的腹部的女性。三烯基前体与HDLp有关。在它们的信息素腺体中，检测到三烯和氘化环氧信息素，表明三烯通过血淋巴运动。实验与男性的飞蛾a s cretacea家蚕显示相同的协会和雌性飞蛾HDLp三烯的局部应用。这一结果表明,成年女性的a . s . cretacea没有出现HDLp专业三烯运输。此外,混合的局部应用包括trienyl前体和另外两个相关的碳氢化合物显示HDLp等量的协会,但选择性的前体在a s cretacea雌性信息素腺。(c) 2006 Elsevier Ltd.版权所有。
Previous experiments with a geometrid species, Ascotis selenaria cretacea, have suggested that a pheromonal C-19 3,4-epoxy-6,9-diene is biosynthesized from the corresponding 3,6,9-triene produced outside a pheromone gland and transported to it via hemolymph after association with lipophorin. In order to clarify this transport, high-density lipophorin (HDLp) in the female moths showing two bands (apoLp I with ca. 250kDa and apoLp II with ca. 80kDa) on an SDS-PAGE was purified by KBr equilibrium density-gradient ultracentrifugation, and the association of the triene was confirmed by GC-MS analysis of a solvent extract from the isolated protein. Next, the role of HDLp was revealed by a topical application of the deuterated trienyl precursor to the abdomens of the females. The trienyl precursor was associated with HDLp. In their pheromone glands, the triene and the deuterated epoxy pheromone were detected, indicating movement of the triene via the hemolymph. Experiments with male moths of A. s. cretacea and female moths of Bombyx mori showed the same association of HDLp with the triene topically applied. This result suggested that the adult females of A. s. cretacea did not develop HDLp specialized in the triene transport. Furthermore, the topical application of a mixture including the trienyl precursor and two other related hydrocarbons showed equal amounts of association by HDLp but selective delivery of the precursor to pheromone glands in the A. s. cretacea females. (c) 2006 Elsevier Ltd. All rights reserved.