Sequences and gene organization of the mitochondrial genomes of the liver flukes Opisthorchis viverrini and Clonorchis sinensis (Trematoda)
Sequences and gene organization of the mitochondrial genomes of the liver flukes Opisthorchis viverrini and Clonorchis sinensis (Trematoda)
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DOI：
10.1007/s00436-011-2477-2
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发表时间：
2012-01-01
影响因子：
2
通讯作者：
Zhu, X. Q.
中科院分区：
文献类型：
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作者：
Cai, X. Q.;Liu, G. H.;Zhu, X. Q.
毒吸睾吸虫（Opisthorchis viverrini）和华支睾吸虫（clon支睾吸虫）是感染人畜的重要吸虫，隶属于吸睾科。本研究对来自老挝的o.v verrini的近完整线粒体（mt） DNA （mtDNA）序列进行了测序，获得了来自中国和韩国的中华绒螯蟹（C. sinensis）的完整mtDNA序列，并揭示了它们的基因注释和基因组组织。产弧菌、中华c.c sinensis（中国分离株）和中华c.c sinensis（韩国分离株）的mtDNA序列大小分别为13510、13879和13877 bp。每个mt基因组包含36个基因，其中12个基因编码蛋白质，2个基因编码rRNA， 20个基因（O. viverrini）或22个基因（C. sinensis）编码tRNA。该基因的序列与肝片吸虫、卫氏并殖吸虫相同，但与血吸虫不同，所有基因的转录方向一致，核苷酸组成高，其中，猪肝吸虫的基因组中a +T的含量分别为59.39%、60.03%和59.99%。采用最大简约法、最大似然法和贝叶斯法对12个蛋白质编码基因的氨基酸序列进行系统发育分析，均显示出不同的类群，具有较高的统计支持度，表明O. viverrini和C. sinensis是姐妹类群。这些数据为研究这两种肝吸虫的分子流行病学和群体遗传学提供了新的mtDNA标记，并对人类和动物中阿片吸虫病和华支睾吸虫病的分子诊断、预防和控制具有重要意义。
Opisthorchis viverrini and Clonorchis sinensis are important trematodes infecting humans and animals, belonging to the family Opisthorchiidae. In the present study, we sequenced the nearly complete mitochondrial (mt) DNA (mtDNA) sequences of O. viverrini from Laos, obtained the complete mtDNA sequences of C. sinensis from China and Korea, and revealed their gene annotations and genome organizations. The mtDNA sequences of O. viverrini, C. sinensis (China isolate), C. sinensis (Korea isolate) were 13,510, 13,879, and 13,877 bp in size, respectively. Each of the three mt genomes comprises 36 genes, consisting of 12 genes coding for proteins, two genes for rRNA, and 20 genes (O. viverrini) or 22 genes (C. sinensis) for tRNA. The gene content and arrangement are identical to that of Fasciola hepatica, and Paragonimus westermani, but distinct from Schistosoma spp. All genes are transcribed in the same direction and have a nucleotide composition high in T. The contents of A+T of the mt genomes were 59.39% for O. viverrini, 60.03% for C. sinensis (China isolate), and 59.99% for C. sinensis (Korea isolate). Phylogenetic analyses using concatenated amino acid sequences of the 12 protein-coding genes, with three different computational algorithms [maximum parsimony, maximum likelihood, and Bayesian analysis], all revealed distinct groups with high statistical support, indicating that O. viverrini and C. sinensis represent sister taxa. These data provide additional novel mtDNA markers for studying the molecular epidemiology and population genetics of the two liver flukes and should have implications for the molecular diagnosis, prevention, and control of opisthorchiasis and clonorchiasis in humans and animals.