Disrupting Glutamate Co-transmission Does Not Affect Acquisition of Conditioned Behavior Reinforced by Dopamine Neuron Activation.
Disrupting Glutamate Co-transmission Does Not Affect Acquisition of Conditioned Behavior Reinforced by Dopamine Neuron Activation.
复制标题
破坏谷氨酸共同传递不会影响多巴胺神经元激活强化的条件行为的获得。
DOI：
10.1016/j.celrep.2017.02.062
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发表时间：
2017
期刊：
影响因子：
8.8
通讯作者：
Ikemoto,Satoshi
中科院分区：
文献类型：
--
作者：
Wang,DongV;Viereckel,Thomas;Zell,Vivien;Konradsson-Geuken,Åsa;Broker,CarlJ;Talishinsky,Aleksandr;Yoo,JiHoon;Galinato,MelissaH;Arvidsson,Emma;Kesner,AndrewJ;Hnasko,ThomasS;Wallén-Mackenzie,Åsa;Ikemoto,Satoshi
腹侧被盖区（VTA）的多巴胺神经元先前被发现表达囊泡谷氨酸转运体2（VGLUT 2），并在腹侧纹状体（VStr）中共同传递谷氨酸。这种能力可能在强化学习中发挥重要作用。尽管已知VTA-VStr多巴胺系统的激活容易强化行为，但对谷氨酸共传递在这种强化中的作用知之甚少。通过结合电极记录和光遗传学，我们发现，在体内刺激VTA多巴胺神经元诱发了许多成年小鼠VStr神经元的快速兴奋反应。而在多巴胺神经元中编码VGLUT 2的基因的条件性敲除在很大程度上消除了快速兴奋性反应，它对多巴胺神经元激活所增强的条件性反应的获得几乎没有影响。因此，谷氨酸共传递似乎是获得DA神经元激活增强的条件反应的先决条件。
Dopamine neurons in the ventral tegmental area (VTA) were previously found to express vesicular glutamate transporter 2 (VGLUT2) and to co-transmit glutamate in the ventral striatum (VStr). This capacity may play an important role in reinforcement learning. Although it is known that activation of the VTA-VStr dopamine system readily reinforces behavior, little is known about the role of glutamate co-transmission in such reinforcement. By combining electrode recording and optogenetics, we found that stimulation of VTA dopamine neurons in vivo evoked fast excitatory responses in many VStr neurons of adult mice. Whereas conditional knockout of the gene encoding VGLUT2 in dopamine neurons largely eliminated fast excitatory responses, it had little effect on the acquisition of conditioned responses reinforced by dopamine neuron activation. Therefore, glutamate co-transmission appears dispensable for acquisition of conditioned responding reinforced by DA neuron activation.