Visual electrochemiluminescence biosensing of aflatoxin M1 based on luminol-functionalized, silver nanoparticle-decorated graphene oxide
Visual electrochemiluminescence biosensing of aflatoxin M1 based on luminol-functionalized, silver nanoparticle-decorated graphene oxide
复制标题
DOI：
10.1016/j.bios.2017.09.035
复制
发表时间：
2018-02-15
影响因子：
12.6
通讯作者：
Mehrgardi, Masoud A.
中科院分区：
文献类型：
--
作者：
Khoshfetrat, Seyyed Mehdi;Bagheri, Hasan;Mehrgardi, Masoud A.
介绍了一种利用封闭双极电极阵列（BPE）检测黄曲霉毒素M1（AFM 1）的电化学发光（ECL）适体传感器。将巯基化的AFM 1适配体固定在金纳米颗粒包覆的磁性Fe 3 O 4纳米颗粒（Apt-GMNP）上。鲁米诺官能化的银纳米颗粒修饰的氧化石墨烯（GO-L-AgNP）参与与固定化适体（Apt-GMNP-GO-L-AgNP）的未配对碱基的π-π相互作用。将Apt-GMNP-GO-L-AgNP引入金阳极BPE阵列后，使各个电极经受不同浓度的AFM 1。在AFM 1与适体相互作用后，GO-L-AgNP从适体上分离;使用光电倍增管（PMT）或智能手机监测阳极处鲁米诺和H2 O2的所得ECL，并使用ImageJ软件分析图像。该过程在阴极极处触发噻吩还原。在面心中心复合设计（FCCD）优化条件下，基于PMT的BPE-ECL适体传感器检测在5 ~ 150 ng mL（-1）的动态范围内呈现线性响应，检测限为0.01 ng mL（-1）。此外，基于智能手机的检测显示ECL图像灰度值与AFM 1靶标的对数浓度之间在10-200 ng mL（-1）范围内呈线性关系，检测限为0.05 ng mL（-1）。此外，BPE-ECL适体传感器成功地用于检测牛奶复合介质中的AFM 1，没有任何严重的干扰，具有可靠的重现性（平均相对标准偏差（RSD = 2.3%））。这种基于智能手机的检测为生物分析开辟了新的视野，不需要训练有素的技术人员进行操作，是一种有前途的即时检测技术。
A sensitive electrochemiluminescence (ECL) aptasensor for aflatoxin M1 (AFM1) detection by a closed bipolar electrode (BPE) array has been introduced. The thiolated AFM1 aptamer was immobilized on gold nanoparticle-coated magnetic Fe3O4 nanoparticles (Apt-GMNPs). Luminol-functionalized silver nanoparticle-decorated graphene oxide (GO-L-AgNPs) participates in pi-pi interactions with the unpaired bases of the immobilized aptamer (Apt-GMNPs-GO-L-AgNPs). After the Apt-GMNPs-GO-L-AgNPs were introduced to a gold anodic BPE array, the individual electrodes were subjected to different concentrations of AFM1. Upon the interaction of AFM1 with the aptamers, the GO-L-AgNPs detach from the aptamer; the resulting ECL of luminol and H2O2 at the anodic poles is monitored using a photomultiplier tube (PMT) or smartphone, and the images are analyzed using ImageJ software. This process triggers thionine reduction at the cathodic poles. Under the optimal conditions obtained by a face-centered central composite design (FCCD), the PMT-based detection of the BPE-ECL aptasensor exhibit a linear response over a wide dynamic range from 5 to 150 ng mL(-1), with a detection limit of 0.01 ng mL(-1). Additionally, smartphone-based detection shows a linear relationship between the ECL image gray value and the logarithmic concentration of the AFM1 target over a range of 10-200 ng mL(-1), with a detection limit of 0.05 ng mL(-1). Furthermore, the BPE-ECL aptasensor was successfully used to detect AFM1 in milk complex media without any serious interferences with reliable reproducibility (average relative standard deviation (RSD = 2.3%)). This smartphone-based detection opens a new horizon for bioanalysis that does not require a trained technician to operate and is a promising technology for point-of-care testing.