Gene-targeting studies of mammalian behavior: Is it the mutation or the background genotype?
Gene-targeting studies of mammalian behavior: Is it the mutation or the background genotype?
复制标题
DOI：
10.1016/s0166-2236(96)20020-7
复制
发表时间：
1996-05-01
影响因子：
15.9
通讯作者：
Gerlai, R
中科院分区：
文献类型：
--
作者：
Gerlai, R
在小鼠中产生无效突变的基因靶向是生物学中一种强大的新工具，它将允许对复杂表型如哺乳动物脑功能以及学习和记忆进行分子解剖。然而，试图解释空突变小鼠中出现的表型变化受到几个警告。例如，以有针对性的方式破坏单个基因的能力可能会导致人们忽视其他基因的影响。忽略遗传背景可能导致对结果的误解：本文将证明，在几项分子神经生物学研究中归因于无效突变的表型异常可能仅仅是背景基因的影响。
Gene targeting to create null mutations in mice is a powerful new tool in biology which will allow the molecular dissection of complex phenotypes such as mammalian brain function, and learning and memory. However, the attempt to interpret the phenotypical changes which arise in null-mutant mice is subject to several caveats. For example, the ability to disrupt a single gene in a targeted manner might lead one to overlook the effects of other genes. Ignoring the genetic background might lead to misinterpretation of results: the present article will demonstrate that the phenotypical abnormalities attributed to the null mutation in several molecular-neurobiological studies could simply result from the effects of background genes.