Inhibition of protein phosphatase 1 and 2A down-regulates beta-tubulin gene expression in Trypanosoma rhodesiense.
Inhibition of protein phosphatase 1 and 2A down-regulates beta-tubulin gene expression in Trypanosoma rhodesiense.
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抑制蛋白磷酸酶 1 和 2A 会下调罗得西亚锥虫中 β-微管蛋白基因的表达。
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发表时间：
1995
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影响因子：
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通讯作者：
J. Donelson
中科院分区：
文献类型：
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作者：
Shiyong Li;J. Donelson
我们研究了冈田酸对罗得氏锥虫β-微管蛋白和原环素基因表达的影响。冈田酸是丝氨酸/苏氨酸蛋白磷酸酶1和2A型的特异性抑制剂。冈田酸被发现在分化血流锥虫的过程中将β-微管蛋白mRNA的稳态水平降低了约5倍，在已建立的原循环锥体中降低了约3倍。未观察到对原环素基因表达的影响。冈田酸对原环锥体中β-微管蛋白基因表达的下调发生在转录后水平。这些结果表明，蛋白磷酸酶1和/或2A活性参与维持非洲锥虫体内β-微管蛋白mRNA的稳定水平。
We studied the effect of okadaic acid, a specific inhibitor of serine/threonine protein phosphatase types 1 and 2A, on the expression of beta-tubulin and procyclin genes in Trypanosoma rhodesiense. Okadaic acid was found to decrease the steady-state level of beta-tubulin mRNA about 5-fold in differentiating bloodstream trypanosomes and about 3-fold in established procyclic trypanosomes. No effect was observed on the expression of the procyclin gene. The down-regulation of beta-tubulin gene expression by okadaic acid in procyclic trypanosomes occurs at the post-transcriptional level. These results demonstrate the involvement of protein phosphatase 1 and/or 2A activity in maintaining the steady-state level of beta-tubulin mRNA in African trypanosomes.