Computer-aided 3-D reconstruction of serially sectioned mouse embryos: its use in integrating anatomical organization.
Computer-aided 3-D reconstruction of serially sectioned mouse embryos: its use in integrating anatomical organization.
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连续切片小鼠胚胎的计算机辅助 3D 重建：其在整合解剖组织中的应用。
DOI：
10.1387/ijdb.9184329
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Duncan Davidson
中科院分区：
文献类型：
--
作者：
Matthew H. Kaufman;R. Brune;Richard A. Baldock;Jonathan B.L. Bard;Duncan Davidson
本文回顾了最近的一个项目，使用计算机辅助的方法，使连续切片的小鼠胚胎（数字鼠标）的三维重建的工作。捕获的图像使用扭曲程序对齐，以便以最小的变形实现相邻部分的几乎完美的对齐。在计算机屏幕上看到的部分实际上是计算机生成的灰度图像，分辨率约为10微米。然后，可以在任何平面上切除重建的胚胎，以在计算机屏幕上模拟尽可能精确地匹配观察者自己的材料。然后可以用不同的颜色绘制各个解剖结构域，并且可以通过查询包含解剖结构和其他信息的文本数据库来选择这些域。此外，现在可以生成与胚胎的其余部分隔离的特定系统的单个解剖学上离散的组件或相关组件组的3-D图像，或者如果需要的话，对照完整胚胎或胚胎的特定部分的“幽灵样”图像。文中给出了该系统在解释维管、肠和近轴中胚层系统中的应用实例，同时也讨论了该方法的优点和缺点。最终的目标是提供从受精到发育后14天的小鼠胚胎的三维重建。完成后，该项目将允许基因表达和细胞谱系数据的准确空间映射到正常小鼠胚胎发育的数字图谱上。
This paper reviews recent work on a project that uses a computer-aided approach for making 3-D reconstructions of serially sectioned mouse embryos (the digital mouse). The captured images are aligned using a warping program so that almost perfect alignment of adjacent sections is achieved with minimal deformation. The sections that are viewed on the computer screen are in fact computer-generated grey-level images with a resolution of about 10 microm. The reconstructed embryo may then be resectioned in any plane to simulate as near as possible an exact match on the computer screen to the viewer's own material. Individual anatomical domains may then be painted in different colors, and these domains may be selected by querying the textual database containing anatomical and other information. Further, it is now possible to generate 3-D images of individual anatomically-discrete components or related sets of components of a particular system in isolation from the rest of the embryo, or, if required, against a 'ghost-like' image of the intact embryo, or specific parts of an embryo. In the article, examples are given of the use of the system in interpreting the vascular, gut and paraxial mesoderm systems, while both the advantages and disadvantages of this approach are also discussed. The eventual aim will be to provide 3-D reconstructions of mouse embryos from fertilization up to 14 days postcoitum of development. When completed, this project will allow the accurate spatial mapping of gene-expression and cell lineage data onto the digital Atlas of normal mouse embryonic development.