A demonstration of cellular interactions during the formation of mesoderm and primordial germ cells in Pleurodeles waltlii.
A demonstration of cellular interactions during the formation of mesoderm and primordial germ cells in Pleurodeles waltlii.
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DOI：
10.1111/j.1432-0436.1985.tb00287.x
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发表时间：
1985-03
期刊：
影响因子：
--
通讯作者：
J. Maufroid;A. Capuron
中科院分区：
文献类型：
--
作者：
J. Maufroid;A. Capuron
分离并培养15天的urodele pleurodeles的八细胞胚胎的动物，植物，背和腹侧囊泡体。四个动物囊泡产生的囊泡由不规则形状的表皮界定。在所有其他外植体中，都会发生中胚层结构的形成，可以解释为在分割过程中，在外胚层动物帽和植物蛋黄质量之间发生的电感相互作用。原始生殖细胞（PGC）在78％的病例中形成了培养胚胎的腹侧一半的病例，仅在单独培养两个腹侧植物性胚泡的情况下发生在48％的情况下。从四个植物囊泡体散发出的外植体中缺乏PGC，这被认为是由于Notochord抑制分化的原因。这一假设已通过培养实验证实，在这种实验中，在通常形成的条件下，添加了年轻胃的推定核抑制剂可以阻止PGC的分化。这些观察结果表明，在尿道中，PGC并不是源于八个细胞阶段隔离的细胞，而是外胚层和内胚层之间后来的电感相互作用的产物。
Animal, vegetal, dorsal and ventral blastomeres of eight-cell embryos of the urodele Pleurodeles waltlii were isolated and cultured for 15 days. The four animal blastomeres produced vesicles delimited by an irregularly shaped epidermis. In all other explants, the formation of mesodermal structures occurred, which can be interpreted as the result of inductive interaction, occurring during segmentation, between the ectodermal animal cap and vegetal yolk mass. Primordial germ cells (PGCs), which formed in 78% of cases when the presumptive ventral half to the embryo was cultured, occurred in only 48% of cases when the two ventral vegetal blastomeres were cultured alone. The absence of PGCs in the explants emanating from the four vegetal blastomeres is thought to have been due to inhibition of differentiation by notochord. This hypothesis has been confirmed by culture experiments in which the addition of presumptive chordomesoderm of young gastrulae prevented the differentiation of PGCs under conditions in which they are normally formed. These observations suggest that, in urodeles, PGCs do not arise from cells segregated as early as the eight-cell stage, but are the product of later inductive interaction between ectoderm and endoderm.