Correlated effluxes of adenine nucleotides, Mg2+ and Ca2+ induced in rat-liver mitochondria by external Ca2+ and phosphate.
Correlated effluxes of adenine nucleotides, Mg2+ and Ca2+ induced in rat-liver mitochondria by external Ca2+ and phosphate.
复制标题
外部 Ca2 和磷酸盐诱导大鼠肝线粒体中腺嘌呤核苷酸、Mg2 和 Ca2 的相关流出。
DOI：
10.1111/j.1432-1033.1981.tb05136.x
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
N. Siliprandi
中科院分区：
文献类型：
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作者：
F. Zoccarato;M. Rugolo;D. Siliprandi;N. Siliprandi
外部介质中无机磷酸盐和 Ca2+ 的存在诱导内源性腺嘌呤核苷酸和 Mg2+ 从大鼠肝线粒体紧密平行地流出。这些流出物 (a) 依赖于 pH 值，并受到解偶联剂、呼吸抑制剂和外部 Mg2+ 的抑制； (b) 被 bongkrekate 完全阻止，但被 atractylate 刺激。 ATP、ADP或AMP各自抑制Ca2+和磷酸盐促进的Mg2+的释放；然而，在寡霉素和 P1,P5-二(腺苷-5')-五磷酸（腺苷酸激酶抑制剂）存在的情况下，只有 ADP 有效。此外，当排出约 50% Mg2+ 时观察到的累积 Ca2+ 的释放会被 bongkrekate 和添加的 Mg2+ 延迟，而白术酸盐会加速释放。所有腺嘌呤核苷酸都对阻止累积的 Ca2+ 外流具有显着作用，但在寡霉素和 P1,P5-二(腺苷-5')-五磷酸存在的情况下，只有 ADP 具有活性。从这些结果我们得出结论，外部磷酸盐诱导的大鼠肝线粒体中 Mg2+、Ca2+ 和腺嘌呤核苷酸的流出是相互关联的，并受到外部 ADP 和 Mg2+ 水平的调节。
The presence of inorganic phosphate and Ca2+ in the external medium induces a closely parallel efflux of both endogenous adenine nucleotides and Mg2+ from rat liver mitochondria. These effluxes are (a) pH-dependent and inhibited by uncouplers, respiration inhibitors and external Mg2+; (b) completely prevented by bongkrekate, but stimulated by atractylate. ATP, ADP or AMP each inhibit the release of Mg2+ promoted by Ca2+ and phosphate; however, in the presence of oligomycin and P1,P5-di(adenosine-5')-pentaphosphate (an inhibitor of adenylate kinase) only ADP is effective. Also the release of accumulated Ca2+ observed when approximately 50% Mg2+ is discharged is retarded by bongkrekate and added Mg2+ whereas it is accelerated by atractylate. All adenine nucleotides have a significant effect in retarding the efflux of accumulated Ca2+ but, in the presence of oligomycin and P1,P5-di(adenosine-5')-pentaphosphate, only ADP is active. From these results we conclude that effluxes of Mg2+, Ca2+ and adenine nucleotide from rat liver mitochondria induced by external phosphate are interconnected and regulated by external ADP and Mg2+ levels.