AUXIN PRODUCTION BY PLANT-PATHOGENIC PSEUDOMONADS AND XANTHOMONADS
AUXIN PRODUCTION BY PLANT-PATHOGENIC PSEUDOMONADS AND XANTHOMONADS
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DOI：
10.1128/aem.53.8.1839-1845.1987
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发表时间：
1987-08-01
影响因子：
4.4
通讯作者：
DUNN, MF
中科院分区：
文献类型：
--
作者：
FETT, WF;OSMAN, SF;DUNN, MF
油菜黄单胞菌病原菌。在添加或不添加l -色氨酸的液体培养基中，比较了引起敏感大豆品种叶细胞肥大的甘氨酸和不引起肥大的非致病菌株产生吲哚化合物的能力，包括植物激素吲哚-3-乙酸（IAA）。另外还测试了几种植物致病性黄单胞菌和假单胞菌的IAA产量，以确定IAA的体外产量是否与诱导宿主组织肥厚生长的能力有关。采用薄层色谱法、高效液相色谱法、紫外光谱法、质谱法、气相色谱-质谱法对培养滤液中的吲哚进行鉴定，并采用高效液相色谱法进行定量分析。当液体培养基中添加l -色氨酸时，所有菌株均产生IAA。在常见的豆科病原体丁香假单胞菌培养滤液中发现IAA含量最高。这是唯一一种在培养基中不添加色氨酸的情况下产生IAA的细菌。在不同菌株的培养滤液中鉴定出的其他吲哚包括吲哚-3-乳酸、吲哚-3-醛、吲哚-3-乙酰胺和n -乙酰色氨酸。假单胞菌和黄单胞菌可以通过存在n -乙酰玻璃酸来区分，而n -乙酰玻璃酸仅在黄单胞菌培养滤液中发现。
Pathogenic strains of Xanthomonas campestris pv. glycines which cause hypertrophy of leaf cells of susceptible soybean cultivars and nonpathogenic strains which do not cause hypertrophy were compared for their ability to produce indole compounds, including the plant hormone indole-3-acetic acid (IAA) in liquid media with or without supplementation with L-tryptophan. Several additional strains of plant-pathogenic xanthomonads and pseudomonads were also tested for IAA production to determine whether in vitro production of IAA is related to the ability to induce hypertrophic growth of host tissues. Indoles present in culture filtrates were identified by thin-layer chromatography, high-performance liquid chromatography, UV spectroscopy, mass spectroscopy, and gas chromatography-mass spectrometry and were quantitated by high-performance liquid chromatography. All strains examined produced IAA when liquid media were supplemented with L-tryptophan. The highest levels of IAA were found in culture filtrates from the common bean pathogen Pseudomonas syringae pv. syringae, and this was the only bacterium tested which produced IAA without addition of tryptophan to the medium. Additional indoles identified in culture filtrates of the various strains included indole-3-lactic acid, indole-3-aldehyde, indole-3-acetamide, and N-acetyltryptophan. Pseudomonads and xanthomonads could be distinguished by the presence of N-acetyvitryptophan, which was found only in xanthomonad culture filtrates.