The development of Ca2+ indicators: a breakthrough in pharmacological research.
The development of Ca2+ indicators: a breakthrough in pharmacological research.
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DOI：
10.1016/j.tips.2004.02.004
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发表时间：
2004-04
影响因子：
13.8
通讯作者：
J. Meldolesi
中科院分区：
文献类型：
--
作者：
J. Meldolesi
自 1979 年开始，荧光 Ca2+ 特异性指示剂作为研究工具的发展彻底改变了跨膜信号传导研究。本文总结了“前 Ca2+ 指示剂”时代的最新技术以及开发捕获在胞质溶胶中的指示剂以研究哺乳动物细胞中 Ca2+ 胞质浓度的基本原理。还讨论了这些研究随后扩展到单细胞水平，以及 Ca2+ 指示剂对信号研究的独特影响，以及引入特定的荧光基因构建体，可提供有关细胞内 Ca2+ 浓度的直接、高分辨率信息。
The development, beginning in 1979, of fluorescent Ca2+-specific indicators as research tools has revolutionized transmembrane signaling studies. In this article, the state of the art in the ‘pre-Ca2+-indicator' era and the rationale for the development of indicators trapped in the cytosol to investigate the cytosolic concentration of Ca2+in mammalian cells are summarized. Subsequent extension of these studies to the level of the single cell, together with the unique impact that Ca2+indicators have had on signaling research and the introduction of specific, fluorescent gene constructs that provide direct, high-resolution information about the intracellular concentration of Ca2+, are also discussed.