Double minutes in human carcinoma cell lines, with special reference to breast tumors.
Double minutes in human carcinoma cell lines, with special reference to breast tumors.
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人类癌细胞系中的双分钟，特别是乳腺肿瘤。
DOI：
10.1093/jnci/62.2.257
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
T. Hsu
中科院分区：
文献类型：
--
作者：
P. Barker;T. Hsu
在一项对人类肿瘤细胞系的调查中，在中期观察到了被称为双分钟(Dm)的含有dna的小颗粒。在未培养的恶性积液、一系列14种乳腺癌细胞系和一种宫颈癌细胞系中检测到DM，文献调查表明DM在人类恶性细胞中可能比先前怀疑的更常见。一些人乳腺癌细胞株显示出高发病率的dm，这使得一系列的细胞化学研究成为可能。Dm与人类染色体常染色区染色一致。与典型的染色体不同，dm既没有C带，也没有CD带，分别代表着丝粒旁的异染色质和着丝粒。观察到dm聚集在染色体末端，单个dm附着在染色体上。这种聚集行为允许DM在没有着丝粒区域的情况下通过细胞分裂。这些结果应该提醒肿瘤细胞遗传学家，他们的材料可能含有较低的未检测到的DM。
Small DNA-containing particles called double minutes (dm) were observed in metaphases during a survey of human tumor cell lines. Detection of dm in uncultured malignant effusions, in a series of 14 breast carcinoma cell lines, and in a cervical carcinoma cell line, and a literature survey indicated that dm may be more common among human malignant cells than previously suspected. Some of the human breast carcinoma cell lines showed a high incidence of dm, which permitted a series of cytochemical studies. The dm stained identically with euchromatic regions of human chromosomes. Unlike typical chromsomes, dm contained neither C-bands nor Cd bands indicative of paracentromeric heterochromatin and centromeres, respectively. The dm were observed to cluster at the ends of chromosomes, and individual dm adhered to chromosomes. This clustering behavior allows dm to pass through cell division in the absence of centromeric regions. These results should alert tumor cytogeneticists to the possibility that their material may contain a low incidence of undetected dm.