ERYTHROPOIESIS IN THE DEVELOPING RAINBOW-TROUT, SALMO-GAIRDNERI-IRIDEUS - HISTOCHEMICAL AND IMMUNOCHEMICAL DETECTION OF ERYTHROPOIETIC ORGANS
ERYTHROPOIESIS IN THE DEVELOPING RAINBOW-TROUT, SALMO-GAIRDNERI-IRIDEUS - HISTOCHEMICAL AND IMMUNOCHEMICAL DETECTION OF ERYTHROPOIETIC ORGANS
复制标题
DOI：
10.1002/jez.1402260311
复制
发表时间：
1983-01-01
影响因子：
--
通讯作者：
YAMAMOTO, M
中科院分区：
文献类型：
--
作者：
IUCHI, I;YAMAMOTO, M
在虹鳟中，S. G.虹膜，圆形，盘状红细胞在胚胎循环（Ery L）被取代的小，椭圆形，盘状红细胞（Ery ImA）孵化后。在alevins的外周血中，Ery ImA生长为大椭圆形、圆盘状的成熟成年红细胞（Ery A）。Ery L和Ery A分别具有幼虫和成虫Hb。通过o-联茴香胺组织化学以及免疫组织化学使用FITC[异硫氰酸荧光素]-特异于成人Hb的抗体探针，研究了红细胞生成过程中Hb转换和红细胞置换的个体发生序列。胚胎红细胞生成的第一阶段（对于Ery L）发生在胚胎内中间细胞团以及卵黄囊上的胚胎外血岛中。红细胞生成的这个阶段是短暂的，在受精后第7 - 12天持续，到第15天（孵化前第5天）停止。在孵化后胚胎的外周血中存在Ery L、ImA和A的混合群。Ery ImA和A与FITC抗Hb A抗体呈免疫荧光反应，而Ery L则无此反应。红系细胞，FITC抗-Hb A抗体染色，观察到肾脏和脾脏孵化前1天，此后，但不是在肝脏中的所有阶段检查。新的红细胞生成（Ery ImA和Ery A的红细胞生成）在孵化前1天在肾脏和脾脏中开始。虹鳟鱼个体发育过程中的血红蛋白转换显然是基于红细胞替代，与红细胞生成部位从一个器官转移到另一个器官相关。
In the rainbow trout, S. g. irideus, round, disc-like erythrocytes in the embryonic circulation (Ery L) are replaced by small, elliptical, disc-like erythrocytes (Ery ImA) after hatching. In the peripheral blood of alevins, Ery ImA grow into mature adult erythrocytes (Ery A) of large elliptical, disc-like shape. Ery L and Ery A have larval and adult Hb, respectively. The ontogenetic sequence of Hb switching and erythrocyte replacement during erythropoiesis was examined by o-dianisidine histochemistry as well as immunohistochemistry using FITC[fluorescein isothiocyanate]-antibody probe specific to adult Hb. The 1st phase of embryonic erythropoiesis (for Ery L) occurs in the intraembryonic intermediate cell mass as well as the extraembryonic blood islands on the yolk sac. This phase of erythropoiesis is transient, continuing during the 7th-12th day after fertilization and ceasing by the 15th day (5th day before hatching). There is a mixed population of Ery L, ImA and A in the peripheral blood of posthatching alevins. Ery ImA and A showed immunofluorescence with FITC anti-Hb A antibody but Ery L did not. Erythroid cells, stainable with FITC anti-Hb A antibody, were observed in the kidney and the spleen 1 day before hatching and thereafter, but not in the liver throughout all stages examined. New erythropoiesis (erythropoiesis for Ery ImA and Ery A) began in the kidney and the spleen 1 day before hatching. Hb switching during the ontogeny of rainbow trout apparently is based on erythrocyte replacement, correlated with a shift in the site of erythropoiesis from one organ to another.