Bioinformatic analysis and functional characterization of the CFEM proteins in maize anthracnose fungus Colletotrichum graminicola
Bioinformatic analysis and functional characterization of the CFEM proteins in maize anthracnose fungus Colletotrichum graminicola
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DOI：
10.1016/s2095-3119(19)62675-4
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发表时间：
2020-02-01
影响因子：
4.8
通讯作者：
Liu Wen-de
中科院分区：
文献类型：
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作者：
Gong An-dong;Jing Zhong-ying;Liu Wen-de
真菌分泌蛋白中含有真菌细胞外膜共有结构域（CFEM），对真菌的致病性有重要作用.玉米炭疽病是由禾生炭疽菌（Colletotrichum graminicola）引起的一种严重病害。在本研究中，我们在C.草生菌。系统发育分析表明，这24个蛋白质（CgCFEM 1 -24）可以分为2个分支的跨膜结构域的基础上的存在。序列比对分析表明，CFEM结构域的氨基酸高度保守，含有8个间隔的半胱氨酸，除了CgCFEM 1和CgCFEM 24分别缺少1个和2个半胱氨酸。十个CgCFEM蛋白与信号肽和没有跨膜结构域被认为是候选效应，因此被选择用于结构预测和功能分析。候选效应子中的CFEM结构域可以形成与白念珠菌中的Csa 2蛋白同源的螺旋篮状结构，其负责血红素获取和致病性。亚细胞定位分析表明，这些效应物积累在细胞膜，细胞核和胞浆体。另外，CgCFM 6、7、8、9和15 5个效应子在有或没有信号肽的情况下可以抑制BAX诱导的本氏烟草中的程序性细胞死亡。这些结果表明，这10个CgCFEM候选效应子具有不同的结构和在宿主细胞中的亚细胞定位，可能在玉米植物的致病过程中发挥重要作用。
Fungal secreted proteins that contain the Common in Fungal Extracellular Membrane (CFEM) domain are important for pathogenicity. The hemibiotrophic fungus Colletotrichum graminicola causes the serious anthracnose disease of maize. In this study, we identified 24 CgCFEM proteins in the genome of C. graminicola. Phylogenic analysis revealed that these 24 proteins (CgCFEM1-24) can be divided into 2 clades based on the presence of the trans-membrane domain. Sequence alignment analysis indicated that the amino acids of the CFEM domain are highly conserved and contain 8 spaced cysteines, with the exception that CgCFEM1 and CgCFEM24 lack 1 and 2 cysteines, respectively. Ten CgCFEM proteins with a signal peptide and without the trans-membrane domain were considered as candidate effectors and, thus were selected for structural prediction and functional analyses. The CFEM domain in the candidate effectors can form a helical-basket structure homologous to the Csa2 protein in Candida albicans, which is responsible for haem acquisition and pathogenicity. Subcellular localization analysis revealed that these effectors accumulate in the cell membrane, nucleus, and cytosolic bodies. Additionally, 5 effectors, CgCFEM6, 7, 8, 9 and 15, can suppress the BAX-induced programmed cell death in Nicotiana benthamiana with or without the signal peptide. These results demonstrate that these 10 CgCFEM candidate effectors with different structures and subcellular localizations in host cells may play important roles during the pathogenic processes on maize plants.