A Method for Direct Demonstration of Pleuropneumonia-Like Organisms in Cultured Cells.∗
A Method for Direct Demonstration of Pleuropneumonia-Like Organisms in Cultured Cells.∗
复制标题
一种在培养细胞中直接证明胸膜肺炎样生物的方法。*
DOI：
10.3181/00379727-117-29731
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发表时间：
1964
影响因子：
--
通讯作者：
H. Fogh
中科院分区：
文献类型：
--
作者：
J. Fogh;H. Fogh
Summary Direct microscopic observation of pleuropneumonia-like organisms (PPLO) in cell cultures is easily accomplished following hypotonic treatment, air-drying and staining with orcein. A rapid technique using FL human amnion cells, inoculated with supernatant from suspected cultures, is described. The demonstration of PPLO contamination of 30 cell lines by this rapid method was in complete agreement with results of PPLO-agar techniques.
