Improved isolation and crystallization of Photosystem I for structural analysis
Improved isolation and crystallization of Photosystem I for structural analysis
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DOI：
10.1016/s0005-2728(98)00059-0
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发表时间：
1998-06-10
影响因子：
4.3
通讯作者：
Witt, HT
中科院分区：
文献类型：
--
作者：
Fromme, P;Witt, HT
利用立体化学纯的β-十二烷基麦芽糖苷从长聚球藻中分离到稳定的三聚体光系统I(PS I)。在低盐浓度下，通过渗析生长出了极纯的PS I晶体。改进的PS I晶体是根据4埃分辨率的X射线结构分析得出的PS I模型的基础(Krauss等人，NA.结构。比奥尔。3(1996)965-973；Schubert等人，J.比奥尔。272(1997)741-769)和关于磁共振技术阐述的电子转移链的详细信息(H.Kass，论文，德国柏林理工大学，1995；Kamlowski等人，Biochim)。生物群落。学报1319(1997年)188-198和199-213；比特尔等人，生物化学36(1997年)12001-12004)。使用微观和宏观晶种的结晶过程被描述为即将到来的结构分析的基础。(C)1998年爱思唯尔科学公司。
Stable trimeric Photosystem I (PS I) was isolated from the cyanobacterium Synechococcus elongatus by use of stereochemically pure beta-dodecylmaltoside. Crystals of extremely pure PS I are grown by dialysis against low salt concentration. The improved PS I crystals were the basis for a PS I model derived from X-ray structure analysis at 4 Angstrom, resolution (Krauss et al., Nat. Struct. Biol. 3 (1996) 965-973; Schubert et al., J. Mel. Biol. 272 (1997) 741-769) and for detailed information on the electron transfer chain elaborated by techniques of magnetic resonance (H. Kass, Thesis, TU-Berlin, 1995; Kamlowski et al., Biochim. Biophys. Acta 1319 (1997) 188-198 and 199-213; Bittl et al., Biochemistry 36 (1997) 12001-12004). Crystallization procedures using micro- and macroseeding are described as basis for the forthcoming structure analysis. (C) 1998 Elsevier Science B.V.