Printed droplet microfluidics for on demand dispensing of picoliter droplets and cells
Printed droplet microfluidics for on demand dispensing of picoliter droplets and cells
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DOI：
10.1073/pnas.1704020114
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发表时间：
2017-08-15
影响因子：
11.1
通讯作者：
Abate, Adam R.
中科院分区：
文献类型：
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作者：
Cole, Russell H.;Tang, Shi-Yang;Abate, Adam R.
虽然生物学的基本单位是细胞，但用于细胞和试剂的微尺度操作的高通量方法是有限的。现有的选择要么是缓慢的，缺乏单细胞特异性，要么使用与细胞不成比例的流体体积。在这里，我们提出了打印液滴微流体技术，一种以确定性控制分配皮升液滴和细胞的技术。其核心技术是一种荧光激活的液滴分选器，它与一种专用基底相结合，共同充当皮升液滴和单细胞打印机，能够高通量地生成复杂的液滴、细胞和微粒阵列。打印液滴微流体技术提供了一种可编程和稳健的技术，用于构建限定细胞和试剂组合的阵列，并将多种测量模式集成在单个测定中。
Although the elementary unit of biology is the cell, high-throughput methods for the microscale manipulation of cells and reagents are limited. The existing options either are slow, lack single-cell specificity, or use fluid volumes out of scale with those of cells. Here we present printed droplet microfluidics, a technology to dispense picoliter droplets and cells with deterministic control. The core technology is a fluorescence-activated droplet sorter coupled to a specialized substrate that together act as a picoliter droplet and single-cell printer, enabling high-throughput generation of intricate arrays of droplets, cells, and microparticles. Printed droplet microfluidics provides a programmable and robust technology to construct arrays of defined cell and reagent combinations and to integrate multiple measurement modalities together in a single assay.