High-resolution mapping of quantitative trait loci for sternopleural bristle number in Drosophila melanogaster.
High-resolution mapping of quantitative trait loci for sternopleural bristle number in Drosophila melanogaster.
复制标题
DOI：
--
复制
发表时间：
1999-08
期刊：
影响因子：
3.3
通讯作者：
M. C. Gurganus;S. Nuzhdin;Jeff Leips;T. F. Mackay
中科院分区：
文献类型：
--
作者：
M. C. Gurganus;S. Nuzhdin;Jeff Leips;T. F. Mackay
我们已经定位了果蝇刷毛数量的数量性状基因座(QTL)，这些基因座包含自然发生的等位基因变异。高(H)、低(L)胸膜刷毛的品系是从一个大的基础群体中人工选择获得的。从选择系中提取等基因H亚系和L亚系，在纯合子低系背景下构建了X和第三染色体H/L重组等基因系群体。Roo转座元件的细胞学位置的多态提供了一个致密的分子标记图谱，平均标记间距离为4.5 cM。采用复合区间作图法，检测到2个X染色体和6个染色体3个QTL影响胸膜毛数选择反应，3个X染色体和3个染色体3个QTL影响腹毛数目相关反应。刷毛数QTL的平均效应较大，有的具有性别特异性效应。影响胸膜刷毛数量的QTL间上位性较常见，互作效应较大。许多含有刷毛数量QTL的区间与先前研究中定位的区间一致。然而，毛数QTL在遗传座位水平上的解析并不是微不足道的，因为含有毛数QTL的基因组区域往往不包含明显的候选基因座，而且对影响成虫毛数的候选基因座突变的定量互补测试结果也是模糊的。
We have mapped quantitative trait loci (QTL) harboring naturally occurring allelic variation for Drosophila bristle number. Lines with high (H) and low (L) sternopleural bristle number were derived by artificial selection from a large base population. Isogenic H and L sublines were extracted from the selection lines, and populations of X and third chromosome H/L recombinant isogenic lines were constructed in the homozygous low line background. The polymorphic cytological locations of roo transposable elements provided a dense molecular marker map with an average intermarker distance of 4.5 cM. Two X chromosome and six chromosome 3 QTL affecting response to selection for sternopleural bristle number and three X chromosome and three chromosome 3 QTL affecting correlated response in abdominal bristle number were detected using a composite interval mapping method. The average effects of bristle number QTL were moderately large, and some had sex-specific effects. Epistasis between QTL affecting sternopleural bristle number was common, and interaction effects were large. Many of the intervals containing bristle number QTL coincided with those mapped in previous studies. However, resolution of bristle number QTL to the level of genetic loci is not trivial, because the genomic regions containing bristle number QTL often did not contain obvious candidate loci, and results of quantitative complementation tests to mutations at candidate loci affecting adult bristle number were ambiguous.