Comparative study of bioconcentration and EROD activity induction in the Japanese flounder, red sea bream, and Java medaka exposed to polycyclic aromatic hydrocarbons
Comparative study of bioconcentration and EROD activity induction in the Japanese flounder, red sea bream, and Java medaka exposed to polycyclic aromatic hydrocarbons
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DOI：
10.1002/tox.20352
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发表时间：
2008-06-01
影响因子：
4.5
通讯作者：
Uno, Seiichi
中科院分区：
文献类型：
--
作者：
Cheikyula, J. Orkuma;Koyama, Jiro;Uno, Seiichi
将日本比目鱼（Paralichthys olivaceus）、真鲷（Pagrus major）和爪哇青鳉（Oryzias javanicus）暴露于水中多环芳烃（PAHs）10 d，比较PAH生物富集和乙氧基试卤灵-O-脱乙基酶（EROD）活性对P450酶的诱导作用，用于亚洲沃茨溢油生物监测。菲、芘和芘的目标接触浓度分别为30微克/升，苯并[a]芘为3.0微克/升。菲和芘积累在比目鱼和红鲷，金只发现在红鲷的肝脏，而爪哇青鳉积累的高分子量苯并[a]芘沿着与其他多环芳烃。总的PAH浓度随着暴露时间的延长而增加，从184 +/- 37纳克/克湿重（w.w.）在第2天至572 +/- 72纳克/克（湿重）在第10天，它，但是，在其他两个物种下降。在这三种鱼类中，爪哇青鳉的PAH初始总浓度最高，为388 +/- 62 ng/g（w.w.）;但最终浓度降至最低，为52.3 +/- 3纳克/克（重量）。它还具有最高的EROD活性4.2 +/- 2.8 nmol/min/mg蛋白质，而在日本比目鱼中最低为0.11 +/- 0.03 nmol/min/mg蛋白质。爪哇青鳉具有较高的EROD活性诱导能力和对所有多环芳烃的生物富集能力，适合于亚洲沃茨多环芳烃的生物监测。由于多环芳烃生物富集度高，还建议考虑将红鲷用于生物监测和多环芳烃慢性毒性试验。(C)2008 Wiley Periodicals，Inc.
Japanese flounder (Paralichthys olivaceus), red sea bream (Pagrus major), and Java medaka (Oryzias javanicus) were exposed to water borne polycyclic aromatic hydrocarbons (PAHs) for 10 days to compare PAH bioconcentration and P450 enzyme induction by ethoxyresorufin-O-deethylase (EROD) activity for use in oil spill biomonitoring in Asian waters. Target exposure concentration for phenanthrene, pyrene, and chrysene were 30 mu g/L each, while benzo[a]pyrene was 3.0 mu g/L. Phenanthrene and pyrene were accumulated in the flounder and red sea bream; chrysene was found only in the livers of the red sea bream, while Java medaka accumulated the high molecular weight benzo[a]pyrene along with the other PAHs. Total PAH concentrations increased with duration of exposure in the red sea bream from 184 +/- 37 ng/g wet weight (w.w.) in day 2 to 572 +/- 72 ng/g (w.w.) in day 10; It, however, decreased in the other two species. Among the three fish species, Java medaka had the highest initial total PAH concentration of 388 +/- 62 ng/g (w.w.); this was, however, reduced to the lowest final concentration of 52.3 +/- 3 ng/g (w.w.). It also had the highest EROD activity of 4.2 +/- 2.8 n mol/min/mg protein compared to the lowest of 0.11 +/- 0.03 n mol/min/mg protein in the Japanese flounder. Java medaka with high EROD activity induction and bioaccumulation of all PAHs will be suitable for PAH biomonitoring in Asian waters. Due to its high PAH bioconcentration red sea bream is also recommended for consideration for biomonitoring and PAH chronic toxicity tests. (C) 2008 Wiley Periodicals, Inc.