Polymorphisms of glutathione S-transferase μ and θ, MDR1 and VEGF genes as risk factors of bladder cancer: A case-control study
Polymorphisms of glutathione S-transferase μ and θ, MDR1 and VEGF genes as risk factors of bladder cancer: A case-control study
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DOI：
10.1016/j.urolonc.2010.08.028
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发表时间：
2012-09-01
影响因子：
2.7
通讯作者：
Valeron, Pilar F.
中科院分区：
文献类型：
--
作者：
Alberto Henriquez-Hernandez, Luis;Navarro, Patricio;Valeron, Pilar F.
目的：探讨西班牙加那利群岛人群中GSTM1、GSTT1、MDR1和血管内皮生长因子(VEGF)基因多态性在膀胱癌发病中的局部分布。材料与方法：采用以医院为基础的病例对照研究方法，对119例膀胱癌患者和110例对照进行基因分型。结果：病例组GSTM1GSTT1阳性54例，阴性46例，对照组65例和35例(P=0.144)，MDR1C3435T，C组57例，T组43例，对照组54例和46例(P=0.633)；病例组VEGFA2578C、(A)40、(C)60例，对照组分别为51例、49例(P=0.221)。在加那利群岛受试者中，经年龄和吸烟习惯调整的多因素分析显示，GSTT1零基因是膀胱癌的显著危险因素(优势比(OR)2.0；95%可信区间(CI)1-3.7；P=0.041)。GSTM1、MDR1C3435T和VEGFA2578C基因多态性在病例组和对照组间的分布差异无统计学意义。在病例组中，患者的初始临床分期、临床分级或复发情况在多态变异之间的分布没有显著差异(P=NS)。结论：在西班牙加那利群岛，GSTT1零基因携带者患膀胱癌的风险可能增加。然而，需要广泛的研究才能准确地证实这些结果。(C)2012 Elsevier Inc.保留所有权利。
Objective: The present study was aimed at examining the local distribution of GSTM1, GSTT1, MDR1, and VEGF gene polymorphisms as possible risk factors contributing to the development of bladder cancer among the population from Canary Islands, Spain.Materials and methods: The genotypes were determined by PCR-based methods in a hospital-based case-control study consisting of 119 cases and 110 controls. The socio-demographic and clinicopathologic data were collected, including the smoking habits of the population covered in the study.Results: The observed allelic frequencies were (%): GSTM1-GSTT1, (positive) 54 and (null) 46 in cases, and 65 and 35, respectively, in controls (P = 0.144); MDR1 C3435T, (C) 57 and (T) 43 in cases, and 54 and 46, respectively, in controls (P = 0.633); VEGF A2578C, (A) 40 and (C) 60 in cases, and 51 and 49, respectively, in controls (P = 0.221). Among Canary Islands subjects, GSTT1-null genotype appeared as a significant risk factor for bladder cancer (odds ratio (OR) 2.0; 95% confidence interval (Cl), 1.0-3.7; P = 0.041), in multivariate analysis adjusted by age and smoking habits. No statistical changes in genotype distribution of GSTM1, MDR1 C3435T, and VEGF A2578C gene polymorphisms were observed between cases and controls. The distribution of the initial clinical stage, clinical grade, or recurrence status was not significantly different among the polymorphic variants in the case group (P = NS).Conclusions: Subjects with the GSTT1-null genotype might be at an increased risk of bladder cancer in Canary Islands, Spain. However, extensive studies are required for accurate confirmation of these results. (C) 2012 Elsevier Inc. All rights reserved.