DELAY OF DISEASE DEVELOPMENT IN TRANSGENIC PLANTS THAT EXPRESS THE TOBACCO MOSAIC-VIRUS COAT PROTEIN GENE
DELAY OF DISEASE DEVELOPMENT IN TRANSGENIC PLANTS THAT EXPRESS THE TOBACCO MOSAIC-VIRUS COAT PROTEIN GENE
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DOI：
10.1126/science.3457472
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发表时间：
1986-05-09
期刊：
影响因子：
56.9
通讯作者：
BEACHY, RN
中科院分区：
文献类型：
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作者：
ABEL, PP;NELSON, RS;BEACHY, RN
将含有烟草花叶病毒(TMV)外壳蛋白(CP)基因克隆的嵌合基因导入烟草细胞，构建在已去除致癌基因的农杆菌Ti载体上。由转化细胞再生的植株表达TMV mRNA和CP作为核性状。将自交的转基因植株接种烟草花叶病毒(TMV)，观察其发病情况。表达CP基因的幼苗症状发育延迟，10%至60%的转基因植物在实验期间没有出现症状。增加接种物中TMV的浓度，可缩短症状出现的时间。这些实验的结果表明，植物可以通过基因转化来抵抗病毒疾病的发展。
A chimeric gene containing a cloned cDNA of the coat protein (CP) gene of tobacco mosaic virus (TMV) was introduced into tobacco cells on a Ti plasmid of Agrobacterium tumefaciens from which tumor inducing genes had been removed. Plants regenerated from transformed cells expressed TMV mRNA and CP as a nuclear trait. Seedlings from self-fertilized transgenic plants were inoculated with TMV and observed for development of disease symptoms. The seedlings that expressed the CP gene were delayed in symptom development and 10 to 60 percent of the transgenic plants failed to develop symptoms for the duration of the experiments. Increasing the concentration of TMV in the inoculum shortened the delay in appearance of symptoms. The results of these experiments indicate that plants can be genetically transformed for resistance to virus disease development.