Phage Therapy of Coral White Plague Disease: Properties of Phage BA3
Phage Therapy of Coral White Plague Disease: Properties of Phage BA3
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DOI：
10.1007/s00284-008-9290-x
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发表时间：
2009-02-01
影响因子：
2.6
通讯作者：
Rosenberg, Eugene
中科院分区：
文献类型：
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作者：
Efrony, Rotem;Atad, Ilil;Rosenberg, Eugene
噬菌体BA 3在珊瑚病原体Thalassomonasloyana中繁殖并溶解。对噬菌体BA 3的全基因组进行了测序，它含有47个开放阅读框，G + C含量为40.9%。噬菌体BA 3在海水中吸附于其饥饿宿主，k = 1.0 × 10（-6）噬菌体ml（-1）min（-1）。在水族馆实验中，当噬菌体与病原体同时或1天后加入时，珊瑚疾病的噬菌体治疗是成功的，但当细菌感染后2天加入时，未能保护珊瑚。当在珊瑚感染后1天加入噬菌体时，噬菌体滴度增加约100倍，并在整个37天的实验中保持存在于水族馆水中。在实验结束时，与珊瑚有关的汞浓度为每平方厘米珊瑚表面2.5 +/-A0.5x10（4）。感染了病原体并用噬菌体处理的珊瑚不会将疾病传播给健康的珊瑚。
The bacteriophage BA3 multiplies in and lyses the coral pathogen Thalassomonas loyana. The complete genome of phage BA3 was sequenced; it contains 47 open reading frames with a 40.9% G + C content. Phage BA3 adsorbed to its starved host in seawater with a k = 1.0 x 10(-6) phage ml(-1) min(-1). Phage therapy of coral disease in aquarium experiments was successful when the phage was added at the same time as the pathogen or 1 day later, but failed to protect the coral when added 2 days after bacterial infection. When the phages were added 1 day after coral infection, the phage titer increased about 100-fold and remained present in the aquarium water throughout the 37-day experiment. At the end of the experiment, the concentration of phages associated with the corals was 2.5 +/- A 0.5 x 10(4) per cm(2) of coral surface. Corals that were infected with the pathogen and treated with phage did not transmit the disease to healthy corals.