A nuclear lamin is required for cytoplasmic organization and egg polarity in Drosophila
A nuclear lamin is required for cytoplasmic organization and egg polarity in Drosophila
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DOI：
10.1038/ncb0901-848
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发表时间：
2001-09-01
影响因子：
21.3
通讯作者：
Krasnow, MA
中科院分区：
文献类型：
--
作者：
Guillemin, K;Williams, T;Krasnow, MA
核纤层是组成核纤层的中间丝-核内膜下的丝状网络-并且是核组装，组织和维护所必需的。在这里，我们提出的证据表明，核纤层蛋白也需要在两个高度极化的细胞类型的细胞质组织。果蝇编码主要核纤层蛋白（Dm（0））基因的合子功能丧失突变破坏了气管系统终末细胞胞质延伸的定向生长。生殖系突变克隆破坏卵母细胞的背腹极性。在突变的卵母细胞中，背侧决定簇Gurken（一种转化生长因子-α同源物）的转录本未能正确定位在卵母细胞核的前背侧表面周围;它们的腹侧扩散导致背侧化的卵，类似于经典背侧化突变squid和fs（1）K10的背侧化卵。核纤层蛋白对细胞质以及核组织的需求对于核纤层蛋白的细胞功能和由核纤层蛋白突变引起的人类疾病的发病机制都具有重要意义。
Nuclear lamins are intermediate filaments that compose the nuclear lamina - the filamentous meshwork underlying the inner nuclear membrane - and are required for nuclear assembly, organization and maintenance. Here we present evidence that a nuclear lamin is also required for cytoplasmic organization in two highly polarized cell types. Zygotic loss-of-function mutations in the Drosophila gene encoding the principal lamin (Dm(0)) disrupt the directed outgrowth of cytoplasmic extensions from terminal cells of the tracheal system. Germline mutant clones disrupt dorsal-ventral polarity of the oocyte. In mutant oocytes, transcripts of the dorsal determinant Gurken, a transforming growth factor-alpha homologue, fail to localize properly around the anterodorsal surface of the oocyte nucleus; their ventral spread results in dorsalized eggs that resemble those of the classical dorsalizing mutations squid and fs(1)K10. The requirement of a nuclear lamin for cytoplasmic as well as nuclear organization has important implications for both the cellular functions of lamins and the pathogenesis of human diseases caused by lamin mutations.