A Subpopulation of Dorsal Lateral/Caudal Ganglionic Eminence-Derived Neocortical Interneurons Expresses the Transcription Factor Sp8
A Subpopulation of Dorsal Lateral/Caudal Ganglionic Eminence-Derived Neocortical Interneurons Expresses the Transcription Factor Sp8
复制标题
背侧/尾侧神经节隆起衍生的新皮质中间神经元亚群表达转录因子 Sp8。
DOI：
10.1093/cercor/bhr296
复制
发表时间：
2012-09-01
期刊：
影响因子：
3.7
通讯作者：
Yang, Zhengang
中科院分区：
文献类型：
--
作者：
Ma, Tong;Zhang, Qiangqiang;Yang, Zhengang
啮齿类动物的皮质GABA能中间神经元起源于皮层下祖细胞，并切向迁移到皮质。虽然大多数小鼠新皮质中间神经元来自内侧和尾侧神经节隆起（分别为MGE和CGE），但仍不清楚外侧神经节隆起（LGE）是否也有助于皮质中间神经元的亚群。在这里，我们表明，转录因子Sp 8表达在五分之一的成年皮层中间神经元，这似乎是来自背侧LGE和背侧CGE（dLGE和dCGE，分别）。与MGE衍生的皮质中间神经元相比，dLGE/dCGE衍生的Sp 8表达（Sp 8+）的中间神经元在胚胎后期出生，峰值产生发生在胚胎第15.5天。它们主要沿沿着脑室下/中间区（SVZ/IZ）路线切向迁移;有些在新生儿皮质SVZ/IZ中继续表达有丝分裂标记物（Ki 67和PH 3）。SP 8+中间神经元继续从SVZ/IZ径向迁移到皮质层在出生后的早期阶段。与MGE衍生的中间神经元相反，dLGE/dCGE衍生的Sp 8+中间神经元遵循由外向内的分层模式，优先占据皮层浅层。
Cortical GABAergic interneurons in rodents originate from subpallial progenitors and tangentially migrate to the cortex. While the majority of mouse neocortical interneurons are derived from the medial and caudal ganglionic eminence (MGE and CGE, respectively), it remains unknown whether the lateral ganglionic eminence (LGE) also contributes to a subpopulation of cortical interneurons. Here, we show that the transcription factor Sp8 is expressed in one-fifth of adult cortical interneurons, which appear to be derived from both the dorsal LGE and the dorsal CGE (dLGE and dCGE, respectively). Compared with the MGE-derived cortical interneurons, dLGE/dCGE-derived Sp8-expressing (Sp8+) ones are born at later embryonic stages with peak production occurring at embryonic day 15.5. They tangentially migrate mainly along the subventricular/intermediate zone (SVZ/IZ) route; some continue to express mitotic markers (Ki67 and PH3) in the neonatal cortical SVZ/IZ. Sp8+ interneurons continue to radially migrate from the SVZ/IZ into the cortical layers at early postnatal stages. In contrast to MGE-derived interneurons, dLGE/dCGE-derived Sp8+ interneurons follow an outside-in layering pattern, preferentially occupying superficial cortical layers.