Molecular cloning, expression, and characterization of chicken IFN-λ
Molecular cloning, expression, and characterization of chicken IFN-λ
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DOI：
10.1089/jir.2007.0117
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发表时间：
2008-01-01
影响因子：
2.3
通讯作者：
Bean, Andrew G. D.
中科院分区：
文献类型：
--
作者：
Karpala, Adam J.;Morris, Kirsten R.;Bean, Andrew G. D.
干扰素（IFN）是脊椎动物抵抗病毒感染的重要第一道防线。此外，IFN-λ，一个最近鉴定的哺乳动物IFN组，已证明在治疗哺乳动物病毒中的抗病毒潜力。随着对禽流感（AI）等疾病的日益关注，迫切需要新的抗病毒策略来管理家禽中的问题病毒。此外，免疫分子如IFN-λ的使用通过增强宿主对病毒的应答为治疗家禽提供了有吸引力的选择。考虑到这一点，我们在这里报告的第一个克隆，表达，并分析鸡干扰素-λ（ChIFN-λ）的生物活性。我们比较了ChIFN-λ与其他物种中鉴定的那些的相似性，并证明ChIFN-λ具有与人IFN-λ（HuIFN-λ）相似的抗病毒特性。我们的研究结果表明，在鸡，在人类中，由ChIFN-lambda证明的抗病毒活性支持其列入针对病毒感染的治疗策略。
Interferons (IFN) provide a critical first line of defense against viral infection in vertebrates. Moreover, IFN-lambda, a recently identified group of mammalian IFN, has demonstrated antiviral potential in the treatment of mammalian viruses. With the growing concern over such diseases as avian influenza (AI), there is a pressing need for new antiviral strategies to manage problem viruses in poultry. Furthermore, the use of immune molecules, such as IFN-lambda, provides an attractive option for treating poultry by augmenting the host response to virus. With this in mind, we report here the first cloning, expression, and analysis of biologic activity of chicken IFN-lambda (ChIFN-lambda). We compared the similarity of ChIFN-lambda to those identified in other species and demonstrate that ChIFN-lambda has antiviral properties similar to those of human IFN-lambda (HuIFN-lambda). Our results demonstrate that in the chicken, as in human, the antiviral activity demonstrated by ChIFN-lambda supports its inclusion in therapeutic strategies directed against viral infections.