Differential marker protein expression specifies rarefaction zone-containing human Adark spermatogonia
Differential marker protein expression specifies rarefaction zone-containing human Adark spermatogonia
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DOI：
10.1530/rep-11-0290
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发表时间：
2012-01-01
期刊：
影响因子：
3.8
通讯作者：
Kirchhoff, Christiane
中科院分区：
文献类型：
--
作者：
von Kopylow, Kathrein;Staege, Hannah;Kirchhoff, Christiane
目前尚不清楚人类A(暗)(Ad)和A(淡)(AP)精原细胞的不同核形态是生殖细胞发育的不同阶段还是有丝分裂周期的不同阶段的表现，或者它们是否反映了未知的分子差异。根据Clermont的经典描述，人类暗A型精原细胞(Ad)可能含有一个、有时两个或三个代表染色质稀疏区的核“空泡”。在免疫组织化学和免疫荧光分析中，这些结构很容易在人类睾丸组织的石蜡切片中被识别出来，因此为我们的研究提供了强有力的形态标记。虽然大多数标记蛋白没有区分有无染色质稀疏区的精原细胞，但双重性别和单抗-3相关转录因子(DMRT1)、酪氨酸激酶受体c-Kit/CD117(KIT)和增殖相关抗原Ki-67(Ki-67)似乎仅限于没有染色质稀疏区的亚型。相反，外体成分10(EXOSC10)被发现积累在稀疏区，这表明该核结构域在RNA加工中可能发挥作用。繁衍(2012)143 45-57
It is unclear whether the distinct nuclear morphologies of human A(dark) (Ad) and A(pale) (Ap) spermatogonia are manifestations of different stages of germ cell development or phases of the mitotic cycle, or whether they may reflect still unknown molecular differences. According to the classical description by Clermont, human dark type A spermatogonium (Ad) may contain one, sometimes two or three nuclear 'vacuolar spaces' representing chromatin rarefaction zones. These structures were readily discerned in paraffin sections of human testis tissue during immunohistochemical and immunofluorescence analyses and thus represented robust morphological markers for our study. While a majority of the marker proteins tested did not discriminate between spermatogonia with and without chromatin rarefaction zones, doublesex-and mab-3-related transcription factor (DMRT1), tyrosine kinase receptor c-Kit/CD117 (KIT) and proliferation-associated antigen Ki-67 (KI-67) appeared to be restricted to subtypes which lacked the rarefaction zones. Conversely, exosome component 10 (EXOSC10) was found to accumulate within the rarefaction zones, which points to a possible role of this nuclear domain in RNA processing. Reproduction (2012) 143 45-57