Role of Autophagy in Angiogenesis Induced by a High-Glucose Condition in RF/6A Cells
Role of Autophagy in Angiogenesis Induced by a High-Glucose Condition in RF/6A Cells
复制标题
自噬在高糖诱导 RF/6A 细胞血管生成中的作用
DOI：
10.1159/000455270
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发表时间：
2017-01-01
期刊：
影响因子：
2.6
通讯作者：
Ma, Le
中科院分区：
文献类型：
--
作者：
Du, Jun-Hui;Li, Xia;Ma, Le
目的：研究D-葡萄糖条件下自噬对RF/6A细胞活力、迁移和管状形成的影响。方法：将培养的RF/6A细胞随机分为4组(对照组、低糖组、高糖组和高糖加3-甲基腺嘌呤[3-MA]组)。检测自噬相关蛋白(ATG7、p62和LC3)的表达。检测细胞活力、细胞迁移、导管形成、活性氧(ROS)生成和细胞凋亡。结果：高糖组细胞活力明显降低，细胞迁移和管状形成明显增加(p<；0.05)。3-MA可显著提高细胞存活率，抑制细胞迁移和管状形成(p<；0.05)。高糖组ROS生成增加，高糖+N-乙酰半胱氨酸组ROS生成减少(p<；0.05)。高糖组细胞凋亡率增加，而高糖+3-MA组细胞凋亡率降低。结论：自噬可能参与了高糖诱导的视网膜新生血管的形成。(C)2017年S.Karger AG，巴塞尔
Purpose: To study the effect of autophagy on vitality, migration, and tube formation of RF/6A cells under the condition of D-glucose. Methods: Cultured RF/6A cells were randomly divided into 4 groups (control, low glucose, high glucose, and high glucose with 3-methyladenine [3-MA]). Autophagy-related proteins (Atg7, p62, and LC3) were monitored. Cell vitality, cell migration, tube formation, reactive oxygen species (ROS) production, and apoptosis were assessed. Results: Cell vitality significantly decreased and cell migration and tube formation significantly increased in the high-glucose group (p < 0.05). Pretreatment with 3-MA significantly increased cell viability and inhibited cell migration and tube formation (p < 0.05). ROS production increased in the high glucose group and decreased in the high-glucose with N-acetylcysteine (NAC) group (p < 0.05). The level of apoptosis increased in the high-glucose group, while it was reduced in the high-glucose with 3-MA group. Conclusion: Autophagy maybe participates in the formation of retinal neovascularization induced by high glucose. (C) 2017 S. Karger AG, Basel